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Safe, Efficient Production of Retroviral Vectors

  • H. Kotani
  • G. J. Mcgarrity
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 137)

Abstract

The first clinical trials in gene therapy were held in the United States in 1990 at the National Institutes of Health (NIH). The first trial was for gene marking. Lymphocytes were removed from a melanoma biopsy, expanded in vitro and transduced with a retroviral vector carrying a gene that conferred resistance to the neomycin analogue, G-418. The clinical objective was to determine the distribution and longevity of these marked lymphocytes. Shortly thereafter, on 14 September 1990, the first clinical trial in gene therapy was initiated. A child with a form of severe combined immune deficiency (SCID), due to a defect in the adenosine deaminase (ADA) gene, was re-infused with her own lymphocytes which had been propagated ex vivo and transduced with the retroviral vector (LASN) that contained the wild-type ADA gene. Results of this and a related ADA trial, which involved the introduction of the gene into CD34+ cells from umbilical-cord blood obtained from neonates, have been published (BLAESE et al. 1995; KOHN et al. 1995).

Keywords

Packaging Cell Severe Combine Immune Deficiency Producer Cell Line Roller Bottle Cell Cube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • H. Kotani
  • G. J. Mcgarrity

There are no affiliations available

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