Skip to main content

Mutation: Nuclear and Plastomic Transformation of Higher Plants Using Microprojectile Bombardment

  • Chapter
Progress in Botany

Part of the book series: Progress in Botany ((BOTANY,volume 60))

  • 330 Accesses

Abstract

Biolistic transformation is the dominant method for construction of transgenic cereals. Although the biolistic approach can be optimized for transformation of any plant (and non-plant) species, the efficiency of generated transformants is significantly lower compared to Agrobacterium-based transformations. Protocols for Agrobacterium-mediated transformation of grasses have been successfully developed during the past years. However, biolistic transformation continues to be of major importance for gene technological advances in cereals and other higher plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahl Goy P, Duesing JH (1995) From pots to plots: genetically modified plants on trial. Bio/Technology 13:454–458

    Article  Google Scholar 

  • Allison LA, Maliga P (1995) Light-responsive and transcription-enhancing elements regulate the plastid psbD core promoter. EMBO J 14:3721–3730

    PubMed  CAS  Google Scholar 

  • Allison LA, Simon LD, Maliga P (1996) Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants. EMBO J 15:2802–2809

    PubMed  CAS  Google Scholar 

  • Bock R, Maliga P (1995) Correct splitting of a group II intron from a chimeric reporter gene transcript in tobacco plastids. Nucleic Acid Res 23:2544–2547

    Article  PubMed  CAS  Google Scholar 

  • Bock R, Kössel H, Maliga P (1994) Introduction of a heterologous editing site into the tobacco plastid genome: the lack of RNA editing leads to a mutant phenotype. EMBO J 13:4623–4628

    PubMed  CAS  Google Scholar 

  • Boynton JE, Gilham NW, Harris EH Hosier JP, Johnson AM, Jones AR, Randolph- Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240:1534–1538

    Article  PubMed  CAS  Google Scholar 

  • Carrer H, Hockenberry TN, Svab Z, Maliga P (1993) Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol Gen Genet 241:49–56

    Article  PubMed  CAS  Google Scholar 

  • Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115:971–980

    PubMed  CAS  Google Scholar 

  • Chilton M-D (1993) Agrobacterium gene transfer: progress on a “poor man’s vector” for maize. Proc Natl Acad Sci USA 90:3119–3120

    Article  PubMed  CAS  Google Scholar 

  • Christou P, Ford TL, Kofron M (1991) Production of transgenic rice (Ozyza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9:957–962

    Article  Google Scholar 

  • Ellis DD, McCabe DE, Mclnnis S, Ramachandran R, Russel DR, Walace KM, Martinell BJ, Roberts DR, Raffia KF, McCown BH (1993) Stable transformation of Picea glauca by particle acceleration. Bio/Technology 11:84–89

    Article  CAS  Google Scholar 

  • Finer JJ, McMullen MD (1990) Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Rep 8:586–589

    Article  Google Scholar 

  • Finnegan J, McElrroy D (1994) Transgenic inactivation: plants fight back! Bio/Technology 12:883–888

    Article  Google Scholar 

  • Golds T, Maliga P, Koop H-U (1993) Stable plastid transformation in PEG-treated protoplasts of Nicotiana tabacum. Biotechnology 11:95–97

    Article  CAS  Google Scholar 

  • Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O’Brien JV, Chambers SA, Adams WR, Willets NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603–618

    Article  PubMed  CAS  Google Scholar 

  • Hansen G, Chilton M-D (1996) “Agrolistic” transformation of plant cells: Integration of T-strands generated in planta. Proc Natl Acad Sci USA 93:14978–14983

    Article  PubMed  CAS  Google Scholar 

  • Hartman CL, Lee L, Day PR, Turner NE (1994) Herbicide resistance turfgrass (Agrostis palustris Huds.) by biolistic transformation. Bio/Technology 12:919–923

    Article  CAS  Google Scholar 

  • Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282

    Article  PubMed  CAS  Google Scholar 

  • Hunold R, Burrus M, Bronner R, Duret J-P, Hahne G (1995) Transient gene expression in sunflower (Helianthus annuus L.) following microprojectile bombardment. Plant Sci 105:95–109

    Article  CAS  Google Scholar 

  • Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T (1996) High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14:745–750

    Article  PubMed  CAS  Google Scholar 

  • Jansson C, Mäenpää P (1997) Site-directed mutangesis for structure-function analysis of the Photosystem II reaction center protein Dl. In: Esser K (ed) Progress in Botany, vol 58. Springer, Berlin Heidelberg New York, pp 352–367

    Google Scholar 

  • Kanevski I, Malinga P (1994) Relocation of the plastid rbcL gene to the nucleus yields functional ribulose-1,5 bisphosphate carboxylase in tobacco chloroplasts. Proc Natl Acad Sci USA 91:1969–1973

    Article  PubMed  CAS  Google Scholar 

  • Lee L (1996) Turfgrass biotechnology. Plant Sci 115:1–8

    Article  Google Scholar 

  • Maier RM, Zeltz P, Kössel H, Bonnard G, Gualberto JM, Grienenberger JM (1996) RNA editing in plant mitochondria and chloroplasts. Plant Mol Biol 32:343–365

    Article  PubMed  CAS  Google Scholar 

  • Maliga P, Carrer H, Kanevski I, Staub J, Svab Z (1993) Plastid engineering in land plants: a conservative genome is open to change. Philos Trans R Soc Lond B 342:203–208

    Article  CAS  Google Scholar 

  • McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6:923–926

    Article  Google Scholar 

  • Moll B, Poslby L, Maliga P (1990) Streptomycin and lincomycin resistances are selective plastid markers in cultured Nicotiana cells. Mol Gen Genet 221:245–250

    Article  CAS  Google Scholar 

  • Nixon P, Jansson C (1996) Cyanobacterial transformation and gene regulation. In: Andersson B, Salter H, Barber J (eds) Molecular genetics of photosynthesis. Oxford Univ Press, Oxford, pp 197–224

    Google Scholar 

  • Pang S-Z, DeBoer DL, Wan Y, Ye G, Layton JG, Neher MK, Armstrong CL, Fry JE, Hinchee AW, Fromm ME (1996) An improved green fluorescence protein gene as a vital marker in plants. Plant Physiol 113:893–900

    Article  Google Scholar 

  • Pyke KA, Leech RM (1992) Chloroplast division and expansion is radically altered by nuclear mutations in Arabidopsis thaliana. Plant Physiol 99:1005–1008

    Article  PubMed  CAS  Google Scholar 

  • Register JC III, Peterson DJ, Bell PJ, Bullock WP, Evans IJ, Frame B, Greenlands AJ, Higgs NS, Jepson I, Jiao S, Lewnau JL, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951–961

    Article  PubMed  CAS  Google Scholar 

  • Reith M, Munholland J (1995) Complete nucleotide sequence of the Phorphyra purpurea chloroplast genome. Plant Mol Biol Rep 13:333–342

    Article  CAS  Google Scholar 

  • Ritala A, Aspegren K, Kurten U, Salmenkallio-Marttila M, Mannonen L, Hannus R, Kauppinen V, Teeri TH, Enari TM (1994) Fertile transgenic barley to particle bombardment of immature embryos. Plant Mol Biol 24:317–325

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D (1995) Chlamydomonas reinhardtii as the photosynthetic yeast. Annu Rev Genet 29:209–230

    Article  PubMed  CAS  Google Scholar 

  • Rochaix J-D (1997) Chloroplast reverse genetics: new insights into the function of plastid genes. Trends Plant Sci 2:419–425

    Article  Google Scholar 

  • Schulze J, Balko C, Zellner B, Koprek T, Hansch R, Nerlich A, Mendel RR (1995) Biolistic transformation of cucumber using embryogenic suspension cultures: long-term expression of reporter genes. Plant Sci 112:197–206

    Article  CAS  Google Scholar 

  • Serrano L, Rochange F, Semblat JP, Marque C, Teulifcres C, Boudet A-M (1996) Genetic transformation of Eucalyptus globulus through biolistics: complementary development of procedures for organogenesis from zygotic embryos and stable transformation of corresponding proliferating tissues. J Exp Bot 47:285–290

    Article  CAS  Google Scholar 

  • Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW (1995) Green-fluorescent protein as a new vital marker in plant cells. Plant J 8:777–784

    Article  PubMed  CAS  Google Scholar 

  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome. EMBO J 5:2043–2049

    PubMed  CAS  Google Scholar 

  • Songstad DD, Somers DA, Griesbach RJ (1995) Advances in alternative DNA delivery techniques. Plant Cell Tissue Org Cult 40:1–15

    Article  CAS  Google Scholar 

  • Staub JM, Maliga P (1993) Accumulation of D1 polypeptide in tobacco plastids is regulated via the untranslated region of the psbA mRNA. EMBO J 12:601–606

    PubMed  CAS  Google Scholar 

  • Staub JM, Maliga P (1994) Translation of psbA mRNA is regulated by light via the 5’ untranslated region in tobacco plastids. Plant J 6:547–553

    Article  PubMed  CAS  Google Scholar 

  • Sugita M, Svab Z, Maliga P, Sugiura M (1997) Targeted deletion of sprA from the tobacco plastid genome indicates that the encoded small RNA is not essential for pre-16S rRNA maturation in plastids. Mol Gen Genet 257:23–27

    Article  PubMed  CAS  Google Scholar 

  • Sugiura M (1996) Structure and replication of chloroplast DNA. In: Andersson B, Salter AH, Barber J (eds) Molecular genetics of photosynthesis. Oxford University Press, Oxford, pp 58–74

    Google Scholar 

  • Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci USA 90:913–917

    Article  PubMed  CAS  Google Scholar 

  • Svab Z, Hajdukiewitz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87:8526–8530

    Article  PubMed  CAS  Google Scholar 

  • Tingay S, McEllroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369–1376

    Article  CAS  Google Scholar 

  • Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10:667–674

    Article  CAS  Google Scholar 

  • Wan Y, Lemaux PG (1994) Generation of large numbers of independantly transformed fertile barley plants. Plant Physiol 104:37–48

    PubMed  CAS  Google Scholar 

  • Wolfe KH, Morden CW, Palmer JD (1992) Function and evolution of a minimal plastid genome from a nonphotosynthetic parasitic plant. Proc Natl Acad Sci USA 89:10648– 10652

    Article  PubMed  CAS  Google Scholar 

  • Ye JS, Sayre RT (1990) Reduction of chloroplast DNA content in Solanum nigrum suspension cells by treatment with chloroplast DNA inhibitors. Plant Physiol 94:1477– 1483

    Article  PubMed  CAS  Google Scholar 

  • Zambryski P (1992) Chronicles from the Agrobacterium-plsmt cell-DNA transfer story. Annu Rev Plant Physiol Mol Biol 43:465–490

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Jansson, C., Mäenpää, P. (1999). Mutation: Nuclear and Plastomic Transformation of Higher Plants Using Microprojectile Bombardment. In: Esser, K., Kadereit, J.W., Lüttge, U., Runge, M. (eds) Progress in Botany. Progress in Botany, vol 60. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59940-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-59940-8_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64189-3

  • Online ISBN: 978-3-642-59940-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics