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Luminescent Lanthanide Chelates for Improved Resonance Energy Transfer and Application to Biology

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Applied Fluorescence in Chemistry, Biology and Medicine

Abstract

Fluorescence resonance energy transfer (FRET) is a technique widely used to measure the distance between two points which are separated by approximately 10–80 Å. Lanthanide-based RET (LRET) is a recent modification of the technique with a number of technical advantages, yet relies on the same fundamental mechanism — subject to careful interpretation of various terms. A number of excellent reviews on FRET have been written [14, 21–28]. A recent review of LRET has also appeared [9], as well as a summary of lanthanide luminescence [29] and its application to bio-assays [30], so here we provide only a brief summary of the relevant theory.

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Selvin, P.R. (1999). Luminescent Lanthanide Chelates for Improved Resonance Energy Transfer and Application to Biology. In: Applied Fluorescence in Chemistry, Biology and Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59903-3_19

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  • DOI: https://doi.org/10.1007/978-3-642-59903-3_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64175-6

  • Online ISBN: 978-3-642-59903-3

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