Abstract
End-labeling is most commonly utilized for synthetic oligodeoxyribo-nucleotides, although double-stranded DNA may also be labeled (particularly if protruding 5′ ends are present). End-labeled oligonucleotides are used for hybridization probes, primer extension analysis, or DNA sequencing using the Maxam-Gilbert chemical cleavage method. End-labeled double- stranded DNA is used in nuclease protection assays and other applications requiring probes of defined length.
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© 1999 Springer-Verlag Berlin Heidelberg
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Igarashi, P. (1999). Radioactive Labeling of DNA and RNA Probes. In: Hildebrandt, F., Igarashi, P. (eds) Techniques in Molecular Medicine. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59811-1_9
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DOI: https://doi.org/10.1007/978-3-642-59811-1_9
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