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Purification of Nucleic Acids From Eukaryotic Cells

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Techniques in Molecular Medicine

Part of the book series: Springer Lab Manual ((SLM))

Abstract

Essential to most molecular biology techniques is the analysis of DNA and RNA. In general, it is difficult to prepare both RNA and high-molecularweight DNA from the same extract, because optimum conditions are mutually exclusive.

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References

  • Aviv H, Leder P (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci USA 69:1408–1412

    Article  PubMed  CAS  Google Scholar 

  • Birnboim HC (1992) Extraction of high molecular weight RNA and DNA from cultured mammalian cells. Methods Enzymol 216:154–160

    Article  PubMed  CAS  Google Scholar 

  • Brawerman G, Mendecki J, Lee SY (1972) A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry 11:637–641

    Article  PubMed  CAS  Google Scholar 

  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochem 162:156–159

    Article  CAS  Google Scholar 

  • Edmonds M, Vaughan Jr MH, Nakazato H (1971) Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci USA 68:1336–1340

    Article  PubMed  CAS  Google Scholar 

  • Gough NM (1988) Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. Analytic Biochem. 173:93–95

    Article  CAS  Google Scholar 

  • Gruber A and Zingales B (1995) One-base double-stranded DNA sequencing using commercial kits. BioTechniques 19:748–750

    PubMed  CAS  Google Scholar 

  • Hoopes BC, McClure WR (1981) Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Res 9:5493–5504

    Article  PubMed  CAS  Google Scholar 

  • Liedke W, Battistini L, Brosnan CF, Raine CS (1994) PCR Methods Applic 3:185–187

    Article  Google Scholar 

  • Okayama H, Berg P (1982) High-efficiency cloning of full-length cDNA. Mol Cell Biol 2:161–170

    PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch, EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Shapiro DJ (1981) Quantitative ethanol precipitation of nanogram quantities of DNA and RNA. Analytical Biochem 110:229–231

    Article  CAS  Google Scholar 

  • Wallace DM (1987) Large-and small-scale phenol extraction. Methods Enzymol 152: 33–41

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Friedhelm Hildebrandt .

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© 1999 Springer-Verlag Berlin Heidelberg

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Nothwang, H.G., Hildebrandt, F. (1999). Purification of Nucleic Acids From Eukaryotic Cells. In: Hildebrandt, F., Igarashi, P. (eds) Techniques in Molecular Medicine. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59811-1_3

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  • DOI: https://doi.org/10.1007/978-3-642-59811-1_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47808-6

  • Online ISBN: 978-3-642-59811-1

  • eBook Packages: Springer Book Archive

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