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cDNA Library Screening

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Techniques in Molecular Medicine

Part of the book series: Springer Lab Manual ((SLM))

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Abstract

cDNA libraries are collections of recombinant DNA sequences, derived from and representing entire mRNA populations present at a given stage of development or differentiation in certain tissues or cell types. cDNA libraries can be self-made and tailored according to individual experimental needs. Alternatively, they can be purchased from a commercial distributor, if the library representing the desired mRNA source is available. In any case, the collection of cDNA sequences is cloned into a vector, a plasmid or phage, allowing the manipulation of the cloned sequences and propagation of the whole library in suitable host bacteria.

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Author information

Authors and Affiliations

  1. Division of Immunology/Hematology, University Children’s Hospital, Steinwiesstraße 75, Zürich, 8032, Switzerland

    Johann Peter Hossle

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  1. Johann Peter Hossle
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Editor information

Editors and Affiliations

  1. Universitäts-Kinderklinik, Mathildenstrasse 1, D-79106, Freiburg, Germany

    Friedhelm Hildebrandt

  2. Section of Nephrology, Dep. of Internal Medicine, Yale University, 2073 Laboratory of Medicine and Pediatrics, 333 Cedar Street, New Haven, Connecticut, 06620-8029, USA

    Peter Igarashi M.D.

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© 1999 Springer-Verlag Berlin Heidelberg

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Hossle, J.P. (1999). cDNA Library Screening. In: Hildebrandt, F., Igarashi, P. (eds) Techniques in Molecular Medicine. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59811-1_19

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  • DOI: https://doi.org/10.1007/978-3-642-59811-1_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47808-6

  • Online ISBN: 978-3-642-59811-1

  • eBook Packages: Springer Book Archive

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