Abstract
Hybridization methods represent standard techniques in molecular biology. In general, they are used to detect particular sequences (target) within a complex mixture of DNA or RNA molecules. DNA or RNA are usually transferred and immobilized to nitrocellulose or, more commonly, to nylon membranes. Complementary single-stranded probes are labeled radioac- tively or non-radioactively. When hybridized to the filter, probes bind to their complementary target sequence via hydrogen bonds. Unhybridized probe is then washed away, and specifically-bound probe is detected by autoradiography or color reaction.
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References
Hames BD, Higgins SJ (ed.) (1985) Nucleic acid hybridisation: a practical approach. IRL Press, Oxford New York Tokyo
Southern, EM (1975) Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis. J Mol Biol 98:503–517
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© 1999 Springer-Verlag Berlin Heidelberg
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Netzer, KO. (1999). Hybridization Methods (Southern and Northern Blotting). In: Hildebrandt, F., Igarashi, P. (eds) Techniques in Molecular Medicine. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59811-1_10
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DOI: https://doi.org/10.1007/978-3-642-59811-1_10
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47808-6
Online ISBN: 978-3-642-59811-1
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