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Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting

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Techniques in Animal Cytogenetics

Part of the book series: Principles and Practice ((PRINCIPLES))

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Abstract

Fluorescence activated analysis and sorting (“flow cytometry”) offers a successful method to characterise particles in a fast, statistically quantitative way (Melamed et al., 1990). Originally developed to analyse and sort cells (Bonner et al., 1972) it is nowadays a highly versatile tool for biomedical research and routine clinical diagnostics (Darzynkiewicz et Crissman, 1990). Flow cytometry was introduced into cytogenetics about 20 years ago (Gray et al., 1975; Stubblefield et al., 1975). “Flow cytogenetics” (Gray and Langlois, 1986; Cremer et al., 1989a) contributed significantly to medicine and molecular biology.

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© 2000 Springer-Verlag Berlin Heidelberg

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Hausmann, M., Cremer, C., Popescu, P., Celeda, D. (2000). Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting. In: Popescu, P., Hayes, H., Dutrillaux, B. (eds) Techniques in Animal Cytogenetics. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59711-4_8

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  • DOI: https://doi.org/10.1007/978-3-642-59711-4_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64095-7

  • Online ISBN: 978-3-642-59711-4

  • eBook Packages: Springer Book Archive

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