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Anti-HIV Ribozyme verschaffen CD4+ T-Zellen in vitro einen Selektionsvorteil

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HIV-Infekt
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Zusammenfassung

Trotz der Erfolge der antiretroviralen Kombinationstherapie bleibt die HIV-Infektion eine unheilbare Erkrankung. Die Entwicklung von Resistenz wie auch die Toxizität sind die wichtigsten Limitationen der antiretroviralen Kombinationstherapie.

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Literatur

  1. Bahner I, Kearns K, Hao QL, Smogorzewska EM, Kohn DB (1996) Transduction of human CD34+ hematopoietic progenitor cells by a retroviral vector expressing an RRE decoy inhibits human immunodeficiency virus type 1 replication in myelomonocytic cells produced in long-term culture. J Virol 70: 4352–4360

    PubMed  CAS  Google Scholar 

  2. Baltimore D. Gene therapy Intracellular immunization (1988) Nature 335: 395–396

    Google Scholar 

  3. Escaich S, Kalfoglou C, Plavec I, Kaushal S, Mosca JD, Bohnlein E (1995) RevM10-mediated inhibition of HIV-1 replication in chronically infected T cells. Hum Gene Ther 6: 625–634

    Article  PubMed  CAS  Google Scholar 

  4. Marasco WA, Haseltine WA, Chen SY (1993) Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc Natl Acad Sci USA 90: 7889–7893

    Article  PubMed  CAS  Google Scholar 

  5. Ojwang JO, Hampel A, Looney DJ, Wong-Staal F, Rappaport J (1992) Inhibition of human immunodeficiency virus type 1 expression by a hairpin ribozyme. Proc Natl Acad Sci USA 89: 10802–10806

    Article  PubMed  CAS  Google Scholar 

  6. Pear WS, Nolan GP, Scott ML, Baltimore D (1993) Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci USA 90: 8392–8396

    Article  PubMed  CAS  Google Scholar 

  7. Perelson AS, Neumann AU, Markowitz M, Leonard JM, Ho DD (1996) HIV-1 dynamics in vivo: virion clearance rate, infected cell life-span, and viral generation time. Science 271: 1582–1586

    Article  PubMed  CAS  Google Scholar 

  8. Rossi JJ. Ribozymes (1992) Curr Opin Biotechnol 3: 3–7

    Article  PubMed  CAS  Google Scholar 

  9. Sarver N, Cantin EM, Chang PS et al. (1990) Ribozymes as potential anti-HIV-1 therapeutic agents. Science 247: 1222–1225

    Article  PubMed  CAS  Google Scholar 

  10. Schmid I, Uittenbogaart CH, Keld B, Giorgi JV (1994) A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J Immunol Methods 170: 145–157

    Article  PubMed  CAS  Google Scholar 

  11. Sczakiel G, Pawlita M (1991) Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA. J Virol 65: 468–472

    PubMed  CAS  Google Scholar 

  12. Sullenger BA, Gallardo HF, Ungers GE, Gilboa E (1990) Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication. Cell 63: 601–608

    Article  PubMed  CAS  Google Scholar 

  13. Wong-Staal F, Poeschla EM, Looney DJ (1998) A controlled, Phase 1 clinical trial to evaluate the safety and effects in HIV-1 infected humans of autologous lymphocytes transduced with a ribozyme that cleaves HIV-1 RNA. Hum Gene Ther 9: 2407–2425

    Article  PubMed  CAS  Google Scholar 

  14. Yamada O, Kraus G, Leavitt MC, Yu M, Wong-Staal F (1994) Activity and cleavage site specificity of an anti-HIV-1 hairpin ribozyme in human T cells. Virology 205: 121–126

    Article  PubMed  CAS  Google Scholar 

  15. Zhou C, Bahner IC, Larson GP, Zaia JA, Rossi JJ, Kohn EB (1994) Inhibition of HIV-1 in human T- lymphocytes by retrovirally transduced anti-tat and rev hammerhead ribozymes. Gene 149: 33–39

    Article  PubMed  CAS  Google Scholar 

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© 2000 Springer-Verlag Berlin Heidelberg

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Klebra, C., Pape, M., Grez, M., Hoelzer, D., Klein, S.A. (2000). Anti-HIV Ribozyme verschaffen CD4+ T-Zellen in vitro einen Selektionsvorteil. In: Brockmeyer, N.H., Hoffmann, K., Reimann, G., Stücker, M., Altmeyer, P., Brodt, R. (eds) HIV-Infekt. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59683-4_14

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  • DOI: https://doi.org/10.1007/978-3-642-59683-4_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64082-7

  • Online ISBN: 978-3-642-59683-4

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