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Internal Ribosome Entry Site-Mediated Translation in Hepatitis C Virus Replication

  • Chapter
The Hepatitis C Viruses

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 242))

Abstract

The initiation of protein translation on eukaryotic messenger RNAs predominantly follows the first AUG rule, as described by KOZAK (1989b). This states that the ribosome begins scanning an RNA molecule from its extreme 5’ end until it encounters an AUG codon, at which point translation is initiated. In eukaryotes, mRNA molecules usually carry an m7GpppG cap structure at their 5’ terminus. This 5’ cap-structure strongly enhances translation, as it facilitates binding of translation initiation factors and the 40S ribosome subunit to the mRNA (reviewed by PAIN 1996; SACHS et al. 1997). The scanning process, or 3’ movement of the 40S ribosome subunit along the RNA in search of an AUG codon, is inhibited by very stable RNA structures. Also, AUG codons positioned between the 5’ terminus and the AUG codon initiating a specific coding region reduce the efficiency of translation.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, 4.104 Medical Research Building, 301 University Boulevard, Galveston, TX, 77555-1019, USA

    R. C. A. Rijnbrand & S. M. Lemon

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  1. R. C. A. Rijnbrand
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  2. S. M. Lemon
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Editors and Affiliations

  1. Division of Digestive Diseases and Genetics — Winship Cancer Center, Emory University School of Medicine, 1639 Pierce Drive, Rm 2101 WMRB, 30322, Atlanta, GA, USA

    Curt H. Hagedorn M.D.

  2. Department of Molecular Microbiology, Washington University School of Medicine, 600 South Euclid Avenue, 63110, St. Louis, MO, USA

    Charles M. Rice M.D.

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© 2000 Springer-Verlag Berlin Heidelberg

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Rijnbrand, R.C.A., Lemon, S.M. (2000). Internal Ribosome Entry Site-Mediated Translation in Hepatitis C Virus Replication. In: Hagedorn, C.H., Rice, C.M. (eds) The Hepatitis C Viruses. Current Topics in Microbiology and Immunology, vol 242. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59605-6_5

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  • DOI: https://doi.org/10.1007/978-3-642-59605-6_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64047-6

  • Online ISBN: 978-3-642-59605-6

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