Abstract
Biological macromolecules are often highly structured and perform complex functions. Due to this complexity, individual macromolecules in a population can differ from each other at any moment in behavior and properties. This variation from molecule to molecule within a population can obscure measurements of structural and functional details. Therefore, the ability to manipulate and characterize individual macromolecules could substantially improve our access to detailed information about molecular structure and function, and is a goal sought avidly during the last three decades. However, analysis of the behavior of single molecules differs fundamentally from that of conventional chemical kinetics: processes involving single molecules are inevitably stochastic because of thermal fluctuations.
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Qian, H., Elson, E.L. (2001). Strain-Dependent Fluorescence Correlation Spectroscopy: Proposing a New Measurement for Conformational Fluctuations of Biological Macromolecules. In: Fluorescence Correlation Spectroscopy. Springer Series in Chemical Physics, vol 65. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59542-4_4
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DOI: https://doi.org/10.1007/978-3-642-59542-4_4
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