Abstract
Human papillomavirus type 16 (HPV 16) is one of the most common high-risk serotypes of human papilloma viruses. Clinical samples containing HPV 16 typically have approximately 1300–53,000 viral genome equivalents per cell [1]; a 40-fold range of viral load. Accurate quantification over this range of initial copy numbers can easily be achieved with several dilutions of the unknown samples and multiple quantification reactions. However, a cost effective means of applying PCR to diagnostic work requires a broad dynamic-range for the assay.
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© 2001 Springer-Verlag Berlin Heidelberg
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Caplin, B.E. (2001). Quantification of Human Papilloma Virus Type 16 Using Quantitative Competitive PCR on the LightCycler. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_6
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DOI: https://doi.org/10.1007/978-3-642-59524-0_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66736-0
Online ISBN: 978-3-642-59524-0
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