Abstract
Herpes simplex virus (HSV) is the most important agent of sporadic encephalitis in industrialized countries. HSV type 1 (HSV-1) is commonly associated with oropharyngeal infections, keratoconjunctivitis, and infections of the central nervous system, whereas HSV-2 commonly produces genital infections that are considered one of the most important sexually transmitted diseases. Neonatal HSV infection following exposure to the virus at delivery can produce severe disseminated infection and death. Powerful therapeutic management exists today, however, antiviral drugs must be administered early. On the other hand, symptoms mimicking HSV infection could lead to the unnecessary application of drugs. Therefore, a rapid and safe method for the detection of HSV appears to be of paramount importance for decreasing the lethality as well as the sequelae of HSV infection.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kessler HH, Pierer K, Weber B, Sakrauski A, Santner B, Stuenzner D, Gergely E, Marth E (1994) Detection of herpes simplex virus DNA from cerebrospinal fluid by PCR and a rapid, nonradioactive hybridization technique. J Clin Microbiol 32: 1881–1886
Larder BA, Kemp SD, Darby G (1987) Related functional domains in virus DNA polymerases. EMBO J 6: 169–175
Tsurumi T, Maeno K, Nishiyama Y (1987) Nucleotide sequence of the DNA polymerase gene of herpes simplex virus type 2 and comparison with the type 1 counterpart. Gene 52: 129–137
Cao M, Xiao X, Egbert T, Darragh TM, Yen TSB (1989) Rapid detection of cutaneous herpes simplex virus infection with the polymerase chain reaction. J Invest Dermatol 92: 391–392
Brice SL, Krzemien D, Weston WL, Huff JC (1989) Detection of herpes simplex virus DNA in cutaneous lesions of erythema multiforme. J Invest Dermatol 93: 183–187
Zandotti C, de Lamballrie X, Guignole-Vignoli C, Bollet C, de Micco P (1993) A rapid DNA extraction method from culture and clinical samples. Suitable for the detection of human cytomegalovirus by the polymerase chain reaction. Acta Virol 37: 106–108
Essary LR, Kinard SJ, Butcher A, Wang H, Laycock KA, Donegan E, McCreedy B, Connell S, Batchelor J, Harris J, Spadoro J, Pepose JS (1996) Screening potential corneal donors for HIV-1 by polymerase chain reaction and a colorimetric microwell hybridization assay. Am J Ophthalmol 122: 526–534
Jaulhac B, Reyrolle M, Sodahlon YK, Jarraud S, Kubina M, Monteil H, Piemont Y, Etienne J (1998) Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR. J Clin Microbiol 36: 2120–2122
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Kessler, H.H. (2001). Qualitative Detection of Herpes Simplex Virus DNA on the LightCycler. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_35
Download citation
DOI: https://doi.org/10.1007/978-3-642-59524-0_35
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66736-0
Online ISBN: 978-3-642-59524-0
eBook Packages: Springer Book Archive