Abstract
The heat stable polymerase, Thermus aquaticus, was first reported for use in the polymerase chain reaction in 1988 (1). Instead of adding the polymerase each cycle, only one addition of enzyme was needed at the beginning of PCR. Once all reaction components were combined, amplification could proceed automatically merely by temperature cycling the sample. Of course, automated thermal cyclers were not available yet, so most laboratories experienced a new incarnation of monotony, the repetitive manual transfer of PCR samples between 3 different water baths. It was clear that there must be a better way.
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Wittwer, C. (2001). Rapid Cycle Real-Time PCR: Methods and Applications. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_1
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DOI: https://doi.org/10.1007/978-3-642-59524-0_1
Publisher Name: Springer, Berlin, Heidelberg
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