Abstract
Detection of point mutations in very small amounts of DNA in our lab is performed in micro dissected material from tumors and small premalignant lesions to assess loss of mutant or wild-type alleles of tumor suppressor genes. Even more demanding is the detection of point mutations in a single cell, which is required in preimplantation genetic diagnosis.
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http://www.nhgri.nih.gov/Intramural_research/Lab_transfer/Bic/
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© 2002 Springer-Verlag Berlin Heidelberg
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Pals, G. (2002). Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes. In: Dietmaier, W., Wittwer, C., Sivasubramanian, N. (eds) Rapid Cycle Real-Time PCR — Methods and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59397-0_9
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DOI: https://doi.org/10.1007/978-3-642-59397-0_9
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63965-4
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