Abstract
The LightCycler** system performs real-time PCR with specific and quantitative analysis of amplified products. All parameters of the PCR reaction have to be carefully adjusted. The most important factors are DNA quality and concentration. Pure DNA is prepared with any manufactured kit. Quantification of genomic DNA samples is essential for accurate quantification of small differences in gene copy numbers or low copy target genes. Classic methods used to quantify genomic DNA have limitations.
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References
Sambrook J, Fritsch EF, Maniatis T (1989) Appendix E: commonly used techniques in molecular cloning. E5: quantitation of DNA and RNA. In: Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Fitzgibbon J, Gillett GT, Woodwark KJ, Boyle JM, Wolfe J, Povey S (1993) Mapping of RXRB to human chromosome 6p21.3. Ann Hum Genet 57: 203–209
Jabs WJ, Hennig H, Fittel M, Pethig K, Smets F, Bucsky P, Kirchner H, Wagner HJ (2001) Normalized quantification by real-time PCR of Epstein Barr virus load in patients at risk for transplant lymphoproliferative disorders. J Clin Microbiol 39: 564–569
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© 2002 Springer-Verlag Berlin Heidelberg
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Pieri-Balandraud, N., Roudier, J., Roudier, C. (2002). Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene. In: Dietmaier, W., Wittwer, C., Sivasubramanian, N. (eds) Rapid Cycle Real-Time PCR — Methods and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59397-0_4
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DOI: https://doi.org/10.1007/978-3-642-59397-0_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63965-4
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