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Präparation von Proteinen für die Gelelektrophorese

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Book cover Proteine: Standardmethoden der Molekular- und Zellbiologie

Part of the book series: Springer Labormanual ((SPRINGER LABOR))

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Zusammenfassung

Proteine sind als direkte Produkte der Genexpression strukturell und funktionell die vielseitigsten und zugleich mengenmäßig häufigsten Makromoleküle (über 50 % des Trockengewichts) der Zelle. Eine typische Säugetierzelle (Hepatocyt) enthält ca. 1010 Proteinmoleküle, die sich auf etwa 104 verschiedene Molekülspezies verteilen. Die verschiedenen Proteine bestimmen einmal Form und Struktur (Strukturproteine) und sind weiterhin entscheidend an Ablauf und Regulation des Stoffwechsels (Enzyme) und aller anderen fundamentalen und spezialisierten Funktionen (wie z. B. Bewegungsvorgänge, intra- und interzellulärer Stofftransport, Signaltransfer innerhalb und zwischen den Zellen, molekulare Erkennungsvorgänge und gewebsspezifische Adhäsion etc.) von Zellen und Geweben eines Organismus beteiligt. Jeder ausdifferenzierte Zelltyp eines vielzelligen Organismus hat daher neben einer Grundausstattung („Haushaltsproteine“) seinen charakteristischen Proteinanteil (zell- bzw. gewebespezifische Proteine).

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Author information

Authors and Affiliations

  1. Abt. Molekulargenetische Diagnostik, Kreuzberger Ring 60, D-65205, Wiesbaden, Germany

    Professor Dr. Werner A. Eckert (Labor Dr. Riegel & Partner)

  2. Deutsches Krebsforschungszentrum, Abteilung für Zellbiologie, Im Neuenheimer Feld 280, D-69120, Heidelberg, Germany

    Jürgen Kartenbeck PD Dr.

Authors
  1. Professor Dr. Werner A. Eckert
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  2. Jürgen Kartenbeck PD Dr.
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© 1997 Springer-Verlag Berlin Heidelberg

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Eckert, W.A., Kartenbeck, J. (1997). Präparation von Proteinen für die Gelelektrophorese. In: Proteine: Standardmethoden der Molekular- und Zellbiologie. Springer Labormanual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59227-0_1

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  • DOI: https://doi.org/10.1007/978-3-642-59227-0_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-47759-1

  • Online ISBN: 978-3-642-59227-0

  • eBook Packages: Springer Book Archive

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