Abstract
The development of new, high sensitivity methods in protein chemistry (microisolation, amino acid microanalysis and microsequencing) has permitted characterization of a large number of previously unknown polypeptides. Initial studies were done with small peptides and have progressed to medium-size polypeptides.
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References
Aebersold RH, Teplow DB, Hood LE, Kent SBH (1986) Electroblotting onto activated glass. Biol Chem 261:4229–4238.
Choli T, Hennig P, Wittmann-Liebold B, Reinhardt R (1988a) Isolation, char-akterization and microsequence analysis of a small basic methylated DNA-binding protein from the Archaebacterium Sulfolobus solfataricus. BBA 950: 193–203.
Choli T, Wittmann-Liebold B, Reinhardt R (1988b) Microsequence analysis of DNA-binding proteins 7a, 7b and 7e from the Archaebacterium Sulfolobus acidocaldarius. J. Biol. Chem. 263: 7087–7093.
Choli T, Kapp U, Wittmann-Liebold B (1990) Blotting of proteins onto Immo-bilon membranes. J Chrom 476: 59–72.
Fimmel S, Choli T., Dencher NA, Buldt G, Wittmann-Liebold B (1989) Topography of surface-exposed amino acids in the membrane protein bacteriorho-dopsin determineted by proteolysis and micro-sequencing. Biochim Biophys Acta 978: 231–240.
Kalghatgi K, Horvath C (1988) Rapid peptide mapping by HPLC. J Chrom 443:343–354.
Kamp RM (1986) High performance liquid chromatography of proteins. In: Wittmann-Liebold B, Salnikow J, Erdmann V (eds) Advanced methods in protein microsequence analysis. Springer, Berlin, Heidelberg, New York, pp 21–33.
Lu H-S, Lai P-H (1986) Use of narrow-bore high performance liquid chromatography for microanalysis of protein structure. J Chrom 368:215–231.
Offord RE (1966) Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature 211: 591–593.
Simpson RJ, Nice EC (1984) In situ cyanogen bromide cleavage of N-terminally blocked proteins in a gas-phase sequencer. Biochem Int 8: 787–791.
Stone KL, Williams KR (1986) High performance liquid chromatographic peptide mapping and amino acid analysis in the subnanomol range. J Chrom 359:203–212.
Vandekerckhove J, Bauw G, Puype M, Van Damm J, Van Montagu M (1985) Protein blotting on polybrene-coated glass-fiber sheets. Eur J Biochem 152:9–19.
Wilson KJ, Huang AL, Brasseur MM, Yuan PN (1986) Microbore high performance liquid chromatography: An example of its applications in peptide characterization. Biochromatography 1:106–112.
Wittmann-Liebold B, Kamp RM (1980) Primary structure of ribosomal protein L29. Biochem Int 1: 436–445.
Wittmann-Liebold B, Lehmann A (1980) New approaches to sequencing by micro-and automatic solid phase technique. In: Birr Ch (ed) Methods in peptide and protein sequence analysis. Elsevier, Amsterdam, pp 49–72
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© 1997 Springer-Verlag Berlin Heidelberg
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Choli-Papadopoulou, T., Kamp, R.M. (1997). Separation of Peptides Using HPLC and TLC Fingerprints. In: Kamp, R.M., Choli-Papadopoulou, T., Wittmann-Liebold, B. (eds) Protein Structure Analysis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59219-5_6
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DOI: https://doi.org/10.1007/978-3-642-59219-5_6
Publisher Name: Springer, Berlin, Heidelberg
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