Abstract
The Edman degradation represents the classical reaction for chemical sequencing of peptides and proteins. In liquid-phase sequencing this reaction is homogeneous; separation of excess reagent and product is achieved by exploitation of the different partition coefficients of two immiscible phases (water/organic). This principle was perfected in the first spinning cup sequencer, designed by Pehr Edman himself. In modern gas-phase sequencers the physical conditions are similar except that the sample is fixed by noncovalent forces to a solid matrix (e.g., Immobilon, Millipore, Eschborn, Germany) and reagents are applied in liquid-or gas -phases. Miniaturization of the whole system in combination with microvolume dosage devices yielded the high sensitivity typical of these instruments.
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Salnikow, J. (1997). Solid-Phase Sequencing of Peptides and Proteins. In: Kamp, R.M., Choli-Papadopoulou, T., Wittmann-Liebold, B. (eds) Protein Structure Analysis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59219-5_11
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DOI: https://doi.org/10.1007/978-3-642-59219-5_11
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