A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method

Choli-Papadopoulou, T.; Skendros, Y.; Katsani, K.

doi:10.1007/978-3-642-59219-5_10

T. Choli-Papadopoulou,
Y. Skendros &
K. Katsani

Part of the book series: Springer Lab Manual ((SLM))

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2 Citations

Abstract

Microsequencing of polypeptides by the Edman degradation technique can be performed either manually or by automated methods (Edman and Begg 1967; Edman and Henschen 1975). The degradation consists of three parts, coupling with 4-N, N′-dimethylaminoazobenzene-4′-isothiocyanate/phenylisothio-cyanate (DABITC/PITC), acid cleavage of the first amino acid as phenylthiazolinone from the peptide chain, and isomerization to the more stable phenylthiohydantoins. The stable derivatives are identified on thin-layer polyamide sheets (Edman and Henschen 1975) or by high performance liquid chromatography (HPLC) (Chang 1988).

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Authors

T. Choli-Papadopoulou
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Y. Skendros
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K. Katsani
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Editors and Affiliations

Technische Fachhochschule Berlin FB3 Chemie- und Biotechnik, Seestraße 64, 13347, Berlin, Germany
Roza Maria Kamp
School of Biochemistry, Laboratory of Biochemistry, Aristotle University Thessaloniki, 54006, Thessaloniki, Greece
Theodora Choli-Papadopoulou
Max-Delbrück-Centrum für Molekulare Medizin, Robert-Rössle-Straße 10, 13125, Berlin-Buch, Germany
Brigitte Wittmann-Liebold

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Choli-Papadopoulou, T., Skendros, Y., Katsani, K. (1997). A Manual Method for Protein Sequence Analysis Using the DABITC/PITC Method. In: Kamp, R.M., Choli-Papadopoulou, T., Wittmann-Liebold, B. (eds) Protein Structure Analysis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59219-5_10

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DOI: https://doi.org/10.1007/978-3-642-59219-5_10
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47765-2
Online ISBN: 978-3-642-59219-5
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