Abstract
During the past decade, biochemical methods of protein purification have become so specialized and sophisticated that it is now difficult for the beginner, whether graduate student or specialist in another field, to follow all the minor but important details which lead to a successful procedure. It should always be kept in mind that there are many alternative ways to purify a particular protein. If a particular procedure is not working another must be tried.
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References
Buse G, Steffens GJ, Steffens GCM, Meinecke K, Hensel S, Reumkens J (1986) Sequence analysis of complex membrane proteins (cytochrome c oxidase). In: B. Wittmann-Liebold et al. (eds) Advanced methods in protein microse-quence analysis. Springer, Berlin Heidelberg, New York, pp 340–351.
Enfords SO (1992) Control of in vivo proteolysis in the production of recombinant proteins. Trends in Biotechnol 10: 310–315.
Furth AJ (1980) Removing unbound detergent from hydrophobic proteins. Anal. Biochem 109, 207–215.
Harris ELV, Angal S (1990) Protein purification applications: a practical approach, IRL, Oxford.
Harris ELV, Angal S (1989) Protein purification applications: a practical approach. IRL, Oxford.
Hoess A, Arthur A K, Wanner G, Fanning E (1988) Recovery of soluble, biologically active recombinant proteins from total bacterial lysates using ion exchange resin. Biotechnology 6:1214–1217.
Marston FLA, Angal S, Lowe PA, Chan M, Hill CR (1988) Scale-up of the recovery and reactivation of recombinant proteins. Biochem Soc Tran. 16: 112–115.
Marston FAO (1986) The purification of eukaryotic polypeptides synthesized in E. coli. Biochemical J 240:1–12.
Penefsky HS, Tzagoloff A (1971) Extraction of water soluble enzymes and proteins from membranes. Methods in Enzymol 22:204–219.
Sassenfeld H M (1990) Engineering protein for purification. Trends Biotechnol 8: 88–93.
Schein CH (1989) Production of soluble recombinant proteins in bacteria. Biotechnology 7:1141–1149.
Scopes RK (1994) Protein purification: principles and practice (3rd edn). Springer, Berlin Heidelberg New York, pp 1–380.
van Kimmenade A, Bond M W, Schumacher JH, Laquoi C, Kastelein RA (1988) Expression, renaturation and purification of recombinant human inter-leukin 4 from E. coli. Eur J Biochem 173, 109–114
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Kyriakidis, D.A. (1997). Purification of Proteins for Sequencing. In: Kamp, R.M., Choli-Papadopoulou, T., Wittmann-Liebold, B. (eds) Protein Structure Analysis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59219-5_1
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DOI: https://doi.org/10.1007/978-3-642-59219-5_1
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