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Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture pp 195–198Cite as

DNA-probing as a tool to monitor the distribution of N2-fixing, denitrifying and nitrifying bacteria in soils

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Part of the book series: NATO ASI Series ((ASIG,volume 39))

Abstract

Microorganisms and plants are believed to significantly contribute to the annual changes in the global N-cycle, but their relative contributions in N2-fixation, denitrification and nitrification are poorly understood as yet. Molecular biological techniques have rarely been applied to identify the percentage of bacteria with such traits in the N-cycle in water or soil ecosystems (e. g. 1,2,3). Recently, this laboratory developed gene probes for all steps of denitrification (with the exception of NO-reduction), nitrification (amoA gene) and N2-fixation (4). To obtain such probes, published sequences of the genes were screened for conserved regions by computer analysis, and oligonucleotide primers were developed of such regions which allowed to amplify gene segments of 400–850 bp by PCR. The amplificates obtained were cloned and sequenced and verified by sequence comparison. The following probes were used:

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Author information

Authors and Affiliations

  1. Botanisches Institut, Universität zu Köln, Gyrhofstr. 15, D-50923, Köln, Germany

    H. Bothe, K. Kloos, K. Kaiser, U. Hüsgen, S. Sonnwald & B. Schmitz

Authors
  1. H. Bothe
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  2. K. Kloos
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  3. K. Kaiser
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  4. U. Hüsgen
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  5. S. Sonnwald
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  6. B. Schmitz
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Editor information

Editors and Affiliations

  1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, PL-61-704, Poznan, Poland

    Andrzej Legocki

  2. Botanisches Institut, Universität zu Köln, Gyrhofstraße 15, D-50923, Köln, Germany

    Hermann Bothe

  3. Lehrstuhl für Genetik, Universität Bielefeld, Postfach 8640, D-35501, Bielefeld, Germany

    Alfred Pühler

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© 1997 Springer-Verlag Berlin Heidelberg

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Bothe, H., Kloos, K., Kaiser, K., Hüsgen, U., Sonnwald, S., Schmitz, B. (1997). DNA-probing as a tool to monitor the distribution of N2-fixing, denitrifying and nitrifying bacteria in soils. In: Legocki, A., Bothe, H., Pühler, A. (eds) Biological Fixation of Nitrogen for Ecology and Sustainable Agriculture. NATO ASI Series, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59112-9_39

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  • DOI: https://doi.org/10.1007/978-3-642-59112-9_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63855-8

  • Online ISBN: 978-3-642-59112-9

  • eBook Packages: Springer Book Archive

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