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Low temperature organic phase biocatalysis using cold-adapted enzymes

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Biotechnological Applications of Cold-Adapted Organisms

Abstract

Enzymes from ectothermic organisms are adapted to function within specific niches in the environment. There seems to be parity in respect of conformational stability, flexibility and activity for enzymes at the growth temperature for the source organisms.1 This makes cold-adapted enzymes particularly suited for catalysis at low temperatures. Organic phase biocatalysis (OPB) refers to the performance of enzymatic reactions in media comprising wholly or partly of organic solvents. This approach extends the well-known advantages of enzymatic catalysis (e.g., high specificity and catalytic efficiency, mild reaction conditions) to reactions involving water insoluble substrates. Replacing an aqueous reaction medium with an organic solvent allows novel reactions, e.g. reverse hydrolysis and transesterification, to be carried out. OPB grew rapidly in the mid-1980’s with applications of enzymes, whole cells and tissues, as biocatalysts in water-organic solvent phase systems.

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Authors and Affiliations

  1. Department of Food Science, School of Physical Sciences, University of Leeds, Leeds, Ls 2 9JT, UK

    R. K. Owusu Apenten

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  1. R. K. Owusu Apenten
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Editors and Affiliations

  1. Institute of Microbiology, University of Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria

    Rosa Margesin  & Franz Schinner  & 

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Apenten, R.K.O. (1999). Low temperature organic phase biocatalysis using cold-adapted enzymes. In: Margesin, R., Schinner, F. (eds) Biotechnological Applications of Cold-Adapted Organisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58607-1_3

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  • DOI: https://doi.org/10.1007/978-3-642-58607-1_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63663-9

  • Online ISBN: 978-3-642-58607-1

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