Abstract
Tissue engineering is a promising new field based on expertise in cell biology, medicine and mechanical engineering. It raises exciting hopes of producing autologous tissue substitutes to replace altered organs. This challenge involves highly specialized technology in order to provide the proper shape to the tissue and promote the maintenance of its native physiological properties. Primary cell populations may lose some of their functional and morphological properties in vitro in the absence of a proper environment. In order to maintain cell integrity, a three-dimensional matrix that mimics the in vivo environment as closely as possible was developed, according to the type of tissue produced [1, 5, 18, 26, 27, 29, 34, 35].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Auger FA, Lòpez Valle CA, Guignard R, Tremblay N, Nöel B, Goulet F, Germain L (1995) Skin equivalents produced using human collagens. In Vitro Cell Dev Biol 31: 432–439
Bell E, Ivarsson B, Merril C (1979) Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA 76:1274–1278
Berthod F, Auger FA (1997) Experimental application of skin substitutes for dermatological purposes. In: Rouabhia M (ed) Skin substitute production by tissue engineering: clinical and fundamental applications. Landes Bioscience, Austin, pp 211–237
Berthod F, Germain L, Guignard R, Lethias C, Garrone R, Damour O, van der Rest M, Auger FA (1997) Differential expression of collagen XII and XIV in human skin and in human reconstructed skin. J Invest Dermatol 108:737–742
Berthod F, Sahuc F, Hayek D, Damour O, Collombel C (1996) Deposition of collagen fibril bundles by long-term culture of fibroblasts in a collagen sponge. J Biomed Mater Res 32: 87–94
Birk DE, Lisenmayer TF (1994) Collagen fibril assembly, deposition, and organization into tissue-specific matrices. In: Yurchenko PD, Birk DE, Mecham PR (eds) Extracellular matrix assembly and structure. Academic Press, San Diego, pp 91–128
Chiquet-Ehrismann R, Tannheimer H, Koch M, Brunner A, Spring J, Martin D, Baumgartner S, Chiquet M (1994) Tenascin-C expression by fibroblasts is elevated in stressed collagen gels. J Cell Biol 127:2093–2101
Cloutier R, Lacasse D, Normand A (1993) ACL reconstruction with LAD: a five-year follow-up. J Bone Joint Surg Br 74 [Suppl III]:273–274
Darby I, Skally O, Gabbiani G (1990) α-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab Invest 63:21–29
Desmoulière A, Geinoz A, Gabbiani F, Gabbiani G (1993) TGFß1 induces α-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 122:103–111
Desmoulière A, Rubbia-Brandt L, Abdiu A, Walz T, Macieira-Coelho A, Gabbiani G (1992) a-smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by γ-interferon. Exp Cell Res 201:64–73
Gabbiani G., Ryan GB, Majno G (1971) Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Experientia 27:549–550
Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR, Majno G (1972) Granulation tissue as a contractile organ. A study of structure and function. J Exp Med 135:719–734
Germain L, Auger FA (1995) Tissue engineered biomaterials: biological and mechanical characteristics. In: Wise DL, Trantolo DJ, Altobelli DE, Yaszemski MJ, Gresser JD, Schwartz ER (eds) Encyclopedic handbook of biomaterials and bioengineering vol 1. Marcel Dekker Inc., New York, pp 699–734
Germain L, Jean A, Auger FA, Garrel DR (1994) Human wound healing fibroblasts have greater contractile properties than dermal fibroblasts. J Surg Res 57:267–273
Goulet F, Germain L, Caron C, Rancourt D, Normand A, Auger FA (1997) Tissue engineered ligament. In: Yahia LH (ed) Ligaments and ligamentoplasties. Springer-Verlag, Berlin Heidelberg New York, pp 367–377
Goulet F, Germain L, Rancourt D, Caron C, Normand A, Auger FA (1997) Tendons and ligaments. In: Lanza R, Langer R, Chick WL (eds) Textbook of tissue engineering. Landes Bioscience, Austin, pp 633–644
Goulet F, Boulet L-P, Chakir J, Tremblay N, Dubé J, Laviolette M, Auger FA (1996) Morphological and functional properties of bronchial cells isolated from normal and asthmatic subjects. Am J Respir Cell Mol Biol 15:312–318
Grinnell F, Lamke R (1984) Reorganization of hydrated collagen lattices by human skin fibroblasts. J Cell Sci 66:51–53
Guidry C, Grinnel F (1985) Studies on the mechanism of hydrated collagen gel reorganization by human fibroblasts. J Cell Sci 79:67–81
Ingber DE (1993) Cellular tensigrity: defining new rules of biological design that govern the cytoskeleton. J Cell Sci 104:613–627
Ingber DE (1998) The architecture of life. Scientific American Jan:48–57
Koch M, Bernasconi C, Chiquet M (1992) A major oligomeric fibroblast proteoglycan identified as a novel form of type-XII collagen. Eur J Biochem 207:847–856
Lafrance H, Guillot M, Germain L, Auger FA (1995) Method for the evaluation of tensile properties of skin equivalents. Med Eng Phys 17:537–543
Lafrance H, Yahia L’H, Germain L, Guillot M, Auger FA (1995) Study of the tensile properties of living skin equivalents. Biomed Mater Eng 5:195–208
L’Heureux N, Germain L, Labbé R, Auger FA (1993) In vitro construction of a human blood vessel from cultured vascular cells: a morphologic study. J Vasc Surg 17:499–509
L’Heureux N, Paquet S, Labbé R, Germain L, Auger FA (1998) A completely biological tissue-engineered human blood vessel. FASEB J 12:47–56
Lambert CA, Soudant EP, Nusgens BV, Lapière CM (1992) Pretranslational regulation of extracellular matrix macromolecules and collagenase expression in fibroblasts by mechanical forces. Lab Invest 66:444–451
López Valle CA, Auger FA, Rompré P, Bouvard V, Germain L (1992) Peripheral anchorage of dermal equivalents. Br J Dermatol 127:365–371
Majno G, Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR (1971) Contraction of granulation tissue in vitro: similarity to smooth muscle. Science 173:548–550
Moulin V, Auger FA, O’Connor-McCourt M, Germain L (1997) Fetal and postnatal sera differentially modulate human dermal fibroblast phenotypic and functional features in vitro. J Cell Phys 171:1–10
Moulin V, Castilloux G, Jean A, Garrel DR, Auger FA, Germain L (1996) In vitro models to study wound healing fibroblasts. Burns 22:359–362
Nishiyama T, McDonough AM, Bruns RR, Burgeson RE (1994) Type XII and XIV collagens mediate interactions between banded collagen fibers in vitro and may modulate extra-cellular matrix deformability. J Biol Chem 269:28193–28199
Paquette JS, Goulet F, Boulet L-P, Tremblay N, Chakir J, Germain L, Auger FA (1998) Three-dimensional production of bronchi in vitro, Can Respir J 5:1
Rompré P, Auger FA, Germain L, Bouvard V, López Valle CA, Thibault J, LeDuy A (1990) The influence of initial collagen and cellular concentrations on the final surface area of dermal and skin equivalents: a Box-Behnken analysis. In Vitro Cell Dev Biol 26:983–990
Schürch W, Seemayer TA, Gabbiani G (1992) Myofibroblast. In: Sternberg SS (ed) Histology for pathologists 5. Raven Press, New York, pp 109–114
Thyberg J, Hedin U, Sjolund M, Palmberg L, Bottger BA (1990) Regulation of differentiated properties and proliferation of arterial smooth muscle cells. Arteriosclerosis 10:966–990
Wang N, Butler JP, Ingber DE (1993) Mechanotransduction across the cell surface and through the cytoskeleton. Science 260:1124–1127
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Auger, F.A., Berthod, F., Goulet, F., Germain, L. (1999). What Is New in Mechanical Properties of Tissue-Engineered Organs. In: Desmoulière, A., Tuchweber, B. (eds) Tissue Repair and Fibrosis. Current Topics in Pathology, vol 93. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58456-5_9
Download citation
DOI: https://doi.org/10.1007/978-3-642-58456-5_9
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63603-5
Online ISBN: 978-3-642-58456-5
eBook Packages: Springer Book Archive