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Transforming Growth Factor-β Induction of α-Smooth Muscle Actin Is Dependent on the Deformability of the Collagen Matrix

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Tissue Repair and Fibrosis

Part of the book series: Current Topics in Pathology ((CT PATHOLOGY,volume 93))

Abstract

Current evidence suggests that fibroblasts undergo phenotypic modulation during several physiologic and pathologic conditions, such as wound healing and fibrocontractive diseases [29]. These phenotypically altered fibroblasts develop cytoskeletal features similar to those of smooth muscle cells (SMCs), including the expression of α-smooth muscle actin (α-SMA), which is a contractile isoform of actin and, hence, they have been denoted as myofibroblasts [8]. The myofibroblast is thought to contribute to the process of wound contraction, as well as contractile phenomena observed during fibrotic diseases. However, little is known about the mechanisms responsible for phenotypic modulation of fibroblasts and the expression of α-SMA. Several types of growth factors and cytokines that are locally released from inflammatory and stromal cells at wound sites, as well as components of the extracellular matrix, have been studied to evaluate their role in fibroblastic differentiation. A likely candidate for regulation of α-SMA is transforming growth factor-β (TGF-β) [7], a potent regulator of matrix remodeling. This regulation may be achieved in part by modulation of cell-matrix interactions.

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References

  1. Arora PD, McCulloch CAG (1994) Dependence of collagen remodeling on a-smooth muscle actin expression by fibroblasts. J Cell Physiol 159:161–175

    Article  PubMed  CAS  Google Scholar 

  2. Bellows CG, Melcher AH, Aubin JE (1981) Contraction and organization of collagen gels by cell cultured from periodontal differences between cell types. J Cell Sci 50:299–314

    PubMed  CAS  Google Scholar 

  3. Bjorkerud S (1991) Effects of transforming growth factor-βl on human arterial smooth muscle cells in vitro. Arterioscler Thromb 11:892–902

    Article  PubMed  CAS  Google Scholar 

  4. Chou DH, Lee W, McCulloch CAG (1996) TNF-alpha inactivation of collagen receptors: implications for fibroblast function and fibrosis. J Immunol 156:4354–4362

    PubMed  CAS  Google Scholar 

  5. Cromack DT, Sporn MB, Roberts AB, Merino MJ, Dart LL, Norton JA (1987) Transforming growth factor β levels in rat wound chambers. J Surg Res 42:622–628

    Article  PubMed  CAS  Google Scholar 

  6. Desmouliere A, Gabbiani G (1994) Modulation of fibroblastic cytoskeletal features during pathological situations: the role of extracellular matrix and cytokines. Cell Motil Cytoskel 29: 195–203

    Article  CAS  Google Scholar 

  7. Desmouliere A, Geiniz A, Gabbiani F, Gabbiani G (1993) Transforming growth factor-β 1 induces α-Smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 122:103–111

    Article  PubMed  CAS  Google Scholar 

  8. Gabbiani G, Ryan GB, Majno G (1971) Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Experientia 27:549–550

    Article  PubMed  CAS  Google Scholar 

  9. Ghosh A, Greenberg ME (1988) Calcium signaling in neurons: molecular mechanisms and cellular consequences. Science 268:239–247

    Article  Google Scholar 

  10. Guhary F, Sachs F (1985) Mechanotransducer ion channels in chick skeletal muscle: the effect of extracellular pH. J Physiol 363:119–134

    Google Scholar 

  11. Gullberg D, Tingstrom A, Thuresson AC, Olsson L, Terracio L, Borg TK, Rubin K (1990) β 1 Integrin-mediated collagen gel contraction is stimulated by PDGF. Exp Cell Res 186:264–272

    Article  PubMed  CAS  Google Scholar 

  12. Hautmann MB, Madsen CS, Owens GK (1997) A transforming growth factor β (TGFβ) control element derives TGF-β-induced stimulation of smooth muscle a-actin gene expression in concert with two CArG elements. J Biol Chem 272:10948–10956

    Article  PubMed  CAS  Google Scholar 

  13. Heino J, Ignotz RA, Hemler ME, Crouse C, Massague J (1989) Regulation of cell adhesion receptors by transforming growth factor-β. Concomitant regulation of integrins that share a common β 1 subunit. J Biol Chem 264:380–388

    PubMed  CAS  Google Scholar 

  14. Klein CE, Dressel D, Steinmayer T, Mauch C, Eckes B, Krieg T, Bankert RB, Weber L (1991) Integrin α 2 β 1 is up-regulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils. J Cell Biol 115:1427–1436

    Article  PubMed  CAS  Google Scholar 

  15. Lansman JB, Hallam TJ, Rink TJ (1987) Single stretch-activated ion channels in vascular endothelial cells as mechanotransducers? Nature 325:811–813

    Article  PubMed  CAS  Google Scholar 

  16. Lawrence DA, Pircher R, Kryceve-Martinerie C, Jullien P (1984) Normal embryo fibroblasts release transforming growth factors in a latent form. J Cell Physiol 121:184–188

    Article  PubMed  CAS  Google Scholar 

  17. Lee W, Sodek J, McCulloch, CAG (1996) Role of integrin in regulation of collagen phagocytosis by human fibroblasts. J Cell Physiol 168:695–704

    Article  PubMed  CAS  Google Scholar 

  18. Lin RY, Sullivan KM, Argenta PA, Meuli M, Lorenz HP, Adzick, NS (1995) Exogenous transforming growth factor-beta amplifies its own expression and induces scar formation in a model of human fetal skin repair. Ann Surg 222:146–154

    Article  PubMed  CAS  Google Scholar 

  19. Lin Y-C, Ho C-H, Grinnell F (1997) Fibroblasts contracting collagen matrices form transient plasma membrane passages through which the cells take up fluorescein isothiocyanatedextran and Ca2+. Mol Biol Cell 8:59–71

    PubMed  CAS  Google Scholar 

  20. McCulloch CAG, Knowles GC (1993) Deficiencies in collagen phagocytosis by human fibroblasts in vitro: a mechanism for fibrosis? J Cell Physiol 155:461–471

    Article  PubMed  CAS  Google Scholar 

  21. Nakagawa S, Pawelek P, Grinnell F (1989a) Extracellular matrix organization modulates fibroblast growth factor responsiveness. Exp Cell Res 182:572–582

    Article  CAS  Google Scholar 

  22. Nakagawa S, Pawelek P, Grinnell F (1989b) Long-term culture of fibroblasts in contracted collagen gels: effect on cell growth and biosysthetic activity. J Invest Dermatol 93:792–798

    Article  CAS  Google Scholar 

  23. Orlandi A, Roppaz P, Gabbiani G (1994) Proliferative activity and α-smooth muscle actin expression in cultured rat aortic smooth muscle cells are differently modulated by transforming growth factor-β 1 and heparin. Exp Cell Res 214:526–536

    Article  Google Scholar 

  24. Paradis P, MacLellan WR, Belaguli NS, Schwartz RJ, Schneider MD (1996) Serum response factor mediates AP-1-dependent induction of the skeletal α-Actin promoter in ventricular myocytes. J Biol Chem 271:10827–10833

    Article  PubMed  CAS  Google Scholar 

  25. Roberts AB, Sporn MB (1989) Regulation of endothelial cell growth, architecture and matrix synthesis by TGF-β. Am Rev Respir Dis 140:1126–1128

    PubMed  CAS  Google Scholar 

  26. Rubenstein PA (1990) The functional importance of multiple actin isoforms. Bioessays 12:309–315

    Article  PubMed  CAS  Google Scholar 

  27. Sappino AP, Schurch W, Gabbiani G (1990) Biology of disease, differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations. Lab Invest 63:144–161

    PubMed  CAS  Google Scholar 

  28. Schiro JA, Chan BMC, Roswit, WT, Kassner PD, Pentland AP, Hemler ME, Eisen AZ, Kupper TS (1991) Integrin α 2 β 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells. Cell 67:403–410

    Article  PubMed  CAS  Google Scholar 

  29. Schmitt-Graff A, Desmouliere A, and Gabbiani G (1994) Heterogenity of myofibroblast phenotypic features: an example of fibroblastic cell plasticity. Virchows Arch 425:3–24

    Article  PubMed  CAS  Google Scholar 

  30. Schurch W, Seemayer TA, Gabbiani G (1992) The myofibroblast. In: SS Sternbery (ed) Histology for pathologists. New York, Raven, pp 109–144

    Google Scholar 

  31. Schwartz MA (1993) Spreading of human endothelial cells on fibronectin or vitronectin triggers elevation of intracellular Ca2+. J Cell Biol 120:1003–1010

    Article  PubMed  CAS  Google Scholar 

  32. Stockbridge LL, French AS (1988) Stretch-activated cation channels in human fibroblasts. Biophys J 54:187–190

    Article  PubMed  CAS  Google Scholar 

  33. Wakefield LM, Smith DM, Masui T, Harris CC, Sporn MB, (1987) Distribution and modulation of the cellular receptor for transforming growth factor-beta. J Cell Biol 105:965–975

    Article  PubMed  CAS  Google Scholar 

  34. Wayner EA, Carter WG (1987) Identification of multiple cell adhesion receptors for type VI collagen and fibronectin in human fibrosarcoma cells possessing unique α and common β-subunits. J Cell Biol 105:1873–1884

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. N. Narani
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  2. P. D. Arora
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  3. A. Lew
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  4. L. Luo
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  5. M. Glogauer
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  6. B. Ganss
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  7. C. A. G. McCulloch
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Editor information

Editors and Affiliations

  1. GREF, Université Victor Segalen Bordeaux 2, 146, Rue Léo-Saignat, 33076, Bordeaux Cedex, France

    Alexis Desmoulière

  2. Départment de Nutrition, Université de Montréal, Pavillon Liliane-de-Stewart C. P. 6128, Succursale Centre-Ville, Montréal, Québec, H3C 3J7, Canada

    Beatriz Tuchweber

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© 1999 Springer-Verlag Berlin Heidelberg

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Narani, N. et al. (1999). Transforming Growth Factor-β Induction of α-Smooth Muscle Actin Is Dependent on the Deformability of the Collagen Matrix. In: Desmoulière, A., Tuchweber, B. (eds) Tissue Repair and Fibrosis. Current Topics in Pathology, vol 93. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58456-5_6

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  • DOI: https://doi.org/10.1007/978-3-642-58456-5_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63603-5

  • Online ISBN: 978-3-642-58456-5

  • eBook Packages: Springer Book Archive

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