Abstract
Inorganic polyphosphate (polyP), an anhydride-linked linear polymer of orthophosphate, is present in a wide variety of organisms from bacteria to mammals (Kulaev 1979; Kulaev and Vegabov 1983; Wood and Clark 1988). Despite the prominence of polyP (as in the vacuolar deposits in yeast that may represent 10 to 20% of cellular dry weight), this molecule has received scant attention. Studies by Kulaev, Wood and a few others (Kulaev et al. 1987; Wood and Clark 1988; Kornberg 1995) disclosed the ubiquity of polyP and identified a few related enzyme activities. Yet, polyP has remained a largely forgotten polymer. Among the reasons is the lack of evidence describing its metabolic role, due in measure to the inadequacy of specific quantitative methods for polyP analysis.
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References
Ahn K, Kornberg A (1990) Polyphosphate kinase from Escherichia coli. Purification and demonstration of a phosphoenzyme intermediate. J Biol Chem 265: 11734–11739
Akiyama M, Crooke E, Kornberg A (1992) The polyphosphate kinase gene of Escherichia coli : isolation and sequence of the ppk gene and membrane location of the protein. J Biol Chem 267: 22556–22561
Akiyama M, Crooke E, Kornberg A (1993) An exopolyphosphatase of Escherichia coli. J Biol Chem 268: 633–639
Ault-Riché D, Fraley CD, Tzeng CM, Kornberg A (1998) Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli. J Bacteriol 180: 1841–1847
Bonting CFC, Kortstee GJJ, Zehnder AJB (1991) Properties of polyphosphate: AMP phosphotransferase of Acinetobacter strain 210A, J Bacteriol 173: 6484–6488
Crooke E, Akiyama M, Rao NN, Kornberg A (1994) Genetically altered levels of inorganic polyphosphate in Escherichia coli. J Biol Chem 269: 6290–6295
Hsieh PC, Shenoy BC, Jentoft JE, Phillips NFB (1993) Purification of polyphosphate and ATP glucose phosphotransferase from Mycobacterium tuberculosis H37Ra: evidence that poly(P) and ATP glucokinase activities are catalyzed by the same enzyme. Protein Exp Purif 4: 76–84
Kornberg SR (1957) Adenosine triphosphate synthesis from polyphosphate by an enzyme from Escherichia coli. Biochim Biophys Acta 26: 294–300
Kornberg A (1995) Inorganic polyphosphate: toward making a forgotten polymer unforgettable. J Bacteriol 177: 491–496
Kornberg A, Kornberg SR, Simms ES (1956) Metaphosphate synthesis by an enzyme from Escherichia coli. Biochim Biophys Acta 20: 215–227
Kulaev IS (1979) The biochemistry of inorganic polyphosphates. Wiley, New York
Kulaev IS, Vegabov VM (1983) Polyphosphate metabolism in microorganisms. Adv Microbiol 15: 731–738
Kulaev IS, Vegabov VM, Shabalin YA (1987) New data on biosynthesis of polyphosphates in yeast. In: Torriani-Gorini A, Rothman FG, Silver A, Wright A, Yagil E (eds) Phosphate metabolism and cellular regulation in microorganisms. American Society for Microbiology, Washington, DC, pp 233–238
Kumble KD, Kornberg A (1995) Inorganic polyphosphate in mammalian cells and tissues. J Biol Chem 270: 5818–5822
Kumble KD, Kornberg A (1996) Endopolyphosphatases for long-chain inorganic polyphosphate in yeast and mammals. J Biol Chem 271: 27146–27151
Kumble KD, Ahn K, Kornberg A (1996) Phosphohistidyl active sites in polyphosphate kinase of Escherichia coll. Proc Natl Acad Sci USA 93: 14391–14395
Kuroda A, Kornberg A (1997) Polyphosphate kinase as a nucleoside diphosphate kinase in Escherichia coli and Pseudomonas aeruginosa. Proc Nat! Acad Sci USA 94: 439–442
Kuroda A, Murphy H, Cashel M, Kornberg A (1997) Guanosine tetra-and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coif. J Biol Chem 272: 21240–21243
Meyer A (1904) Orientierende Untersuchungen über Verbreitung, Morphologie, und Chemie des Volutins. Bot Z 62: 113–152
Rao NN, Liu S, Kornberg A (1998) Inorganic polyphosphate in Escherichia coli. I. The phosphate regulon and the stringent response. J Bacteriol 180: 2186–2193
Shiba T, Tsutsumi K, Yano H, Ihara Y, Kameda A, Tanaka K, Takahashi H, Munekata M, Rao NN, Kornberg A (1997) Inorganic polyphosphate and the induction of rpoS expression. Proc Natl Acad Sci USA 94: 11210–11215
Wiame JM (1947) Yeast metaphosphate. Fed Proc 6: 302
Wood HG, Clark JE (1988) Biological aspects of inorganic polyphosphates. Annu Rev Biochem 57: 235–260
Wurst H, Kornberg A (1994) A soluble exopolyphosphatase of Saccharomyces cerevisiae : purification and characterization. J Biol Chem 269: 10996–11001
Wurst H, Shiba T, Kornberg A (1995) The gene for a major exopolyphosphatase of Saccharomyces cerevisiae. J Bacteriol 177: 898–906
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Ault-Riché, D., Kornberg, A. (1999). Definitive Enzymatic Assays in Polyphosphate Analysis. In: Schröder, H.C., Müller, W.E.G. (eds) Inorganic Polyphosphates. Progress in Molecular and Subcellular Biology, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58444-2_12
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DOI: https://doi.org/10.1007/978-3-642-58444-2_12
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