Skip to main content

Genetic Transformation of Astragalus Species

  • Chapter
Transgenic Medicinal Plants

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 45))

Abstract

The genus Astragalus (Fabaceae family) comprises ca. 2000 species and has a wide distribution. Various aspects of chemical constituents, pharmacology, and in-vitro culture studies have been recently reviewed by the author (see Ionkova 1995).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Alania MD (1985) Cycloartanic triterpenoids of plants. Rast it Resur 4:510–523

    Google Scholar 

  • Asamizu T, Akiyama K, Yasuda I (1990) Aminobutyric acid production by hairy root culture in Astragalus membranaceus. Shoyakugaku Zasshi 44(4):311–315

    CAS  Google Scholar 

  • Cardarelli M, Spanol L, De Paolis A, Mauro ML, Nitali G, Constantino P (1985) Identification of the genetic locus responsible for non-polar root induction by Agrobacterium rhizo genes 1855. Plant Mol Biol 5:385–391

    Article  CAS  Google Scholar 

  • Chu DT, Lepe-Zuniga J, Wong WL, La Pushin R, Mavligit GM (1988) Fractionated extract of Astragalus menbranaceus, a Chinese medicinal herb, potentiates LAK cell cytotoxicity generated by a low dose of recombinant interleukin-2. J Clin Lab Immunol 26(4):183–787

    PubMed  CAS  Google Scholar 

  • Corry JP, Reed LW, Curtis WR (1993) Enhanced recovery of solavetivone from Agrobacterium-transformed root cultures of Hyoscyamus muticus using integrated product extraction, Biotechnol Bioeng 42:503–508

    Article  PubMed  CAS  Google Scholar 

  • Cristen P, Roberts MF (1993) Transformation in Datura species. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 22, Plant Protoplasts and genetic engineering III, Springer, Berlin Heidelberg New York, pp 157–171

    Google Scholar 

  • Dorling PR, Colegate SM, Huxtable CR (1989) Toxic species of the plant genus Swainsona. In: James LF, Elbein AD, Molyneux RJ, Warren CA (eds) Swainsonine and related glycosidase inhibitors. Iowa State University Press, Ames, Iowa, pp 14–22

    Google Scholar 

  • Elbein AD, Molyneux RJ (1987) The chemistry and biochemistry of simple indolisidine and related polyhydroxy alkaloids. In: Pelletier SW (ed) Alkaloids: chemical and biological perspectives, vol 5. John Wiley, New York, pp 1–54

    Google Scholar 

  • Ermayanti TM, McComb JA, Obrien PA (1994) Production of swainsonine in root cultures of Swainsona galedifolia. J Exp Bot 45:633–637

    Article  CAS  Google Scholar 

  • Gubanev I, Krilova I, Tichonova V (1976) Astragalus. In: Agrar plants in the USSR. Misl, Moscow, pp 193–202

    Google Scholar 

  • Hirotani M, Zhou Y, Rui H, Furuya T (1994a) Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus. Phytochemistry 36:665–669

    Article  CAS  Google Scholar 

  • Hirotani M, Zhou Y, Rui H, Furuya T (1994b) Cycloartane triterpene glycosides from the hairy root cultures of Astragalus membranaceus. Phytochemistry 37(5):1403–1407

    Article  CAS  Google Scholar 

  • Hohenschutz ED, Bell EA, Jewess PJ, Leworthy DP, Pryce RJ, Arnold E, Clardy J (1981) Castanospermine, a 1,6,7,8-tetrahydroxyoctahydroindolizidine alkaloid from seeds of Castanospermum australe. Phytochemistry 20:811–814

    Article  CAS  Google Scholar 

  • Hu Z, Alfermann AW (1993) Diterpenoid production in hairy root cultures of Salvia miltiorrhiza. Phytochemistry 32(3):699–703

    Article  CAS  Google Scholar 

  • Ionkova I (1983) Phytochemical investigation of saponins, sapogenins and flavonoids species of the Fabaceae family. Diss, Medical Academy, Fac Pharm, Sofia. Bulgaria

    Google Scholar 

  • Ionkova (1991) Production of saponins by conventional and transformed root cultures of Astragalus hamosus(Fabaceae). Probl Pharm Pharmacol Sofia 5:31–37

    Google Scholar 

  • Ionkova I (1992) A method for rapid accumulation of saponin producing biomass in callus and suspension cultures of Astragalus hamosus. Bulg Authorship Cert N 94 913/29.07.1991

    Google Scholar 

  • Ionkova I (1993) Cycloartane-derived saponins from transformed root cultures of Astragalus membranaceus Bge. ASP, 34th Annu Meet, 18–22 July, San Diego. California

    Google Scholar 

  • Ionkova I (1995) Astragalus species (milk vetch): in vitro culture and production of saponins, astragaline, and other biologically active compounds. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 33 Medicinal and aromatic plants VIII. Springer, Berlin Heidelberg New York, PP 97–138

    Google Scholar 

  • Ionkova I, Alfermann AW (1990) Transformation of Astragalus species by Agrobacterium rhizogenes and their saponin production. Planta Med 56:634

    Article  Google Scholar 

  • Ionkova I, Alfermann AW (1994) Althaea officinalis L. (marshmallow): in vitro cultures and production of biologically active compounds. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 28. Medicinal and aromatic plants VII. Springer, Berlin Heidelberg New York, pp 13–42

    Google Scholar 

  • Ionkova I, Hu Z, Alfermann A (1993) Polysaccharide production by hairy root cultures of some higher medicinal plants. In: Narstead A (ed) 41st Annu Congr Med Plant Res, Dusseldorf. Thieme, Stuttgart. p 85

    Google Scholar 

  • Isa T (1991) Mucilage production by hairy root cultures of Astragalus gummifer. In: Komamine A, Misawa M, Dicosmo F. (eds) Plant cell culture in Japan CMC Co, Copyright, Japan, pp 99–103

    Google Scholar 

  • Lsaev MI, Gorovitz AK, Abubakirov HK (1989) Progress in chemistry of cycloartanes. Chim Prirod Soedin 2:156–157

    Google Scholar 

  • Kitagawa L Wang HK, Tagaki A, Fuchida M, Miura I, Yishikawa (1983) Saponin and sapogenol. XXXV. Chemical constituents of Radix Astragali (A. membranaceus Bge). Astragalosides 1,11.1V, Acetylastragalosid and Isoastragalosides I and II. Chem Pharm Bull 31(2):698–708

    Article  CAS  Google Scholar 

  • Michael JP (1995) Indolizidine and quinolizidine alkaloids. Nat Prod Rep: 535–552

    Google Scholar 

  • Molyneux RJ, James LF, Panter KE, Ralphs MH (1991) Analysis and distribution of swainsonine and related polyhydroxyindolizidine alkaloids by thin layer chromatography. Phytochem Anal 2:125–129

    Article  CAS  Google Scholar 

  • Nikolov S, Ionkova I, Panova D, Budziliewicz H, Sroeder E (1985) Soyasapogenol B from Astragalus angustifolius. CR Acad Bulg Sci 38(7):875–979

    CAS  Google Scholar 

  • Rhodes MJC, Robins JR, Hamil JD, Paar AJ, Walton NJ (1987) Secondery product formation using Agrobacterium rhizogenes transformed “hairy roots” cultures. Newsletter N53:2–15

    Google Scholar 

  • Rizk A (1986) Astragalus. In: Rizk A (ed) The phytochemistry of the flora of Qatar, A Copyright, Qatar, pp 246–254

    Google Scholar 

  • Shimomura K, Sauerwein M, Ishimaru K (1991) Tropane alkaloids in the adventitious and hairy root cultures of solanaceous plants. Phytochemistry 30:2275–2278

    Article  CAS  Google Scholar 

  • Sign MW, Flores HE (1990) The biosynthetic potential of plant roots. Bioessay 12 (N1):7–13

    Article  Google Scholar 

  • Staba JE (1985) Milestones in plant tissue culture systems for the production of secondary products. J Nat Prod 48(2):203–209

    Article  CAS  Google Scholar 

  • Stepashkina KI (1959) Astragalus and their application in clinic practice. Gandjia IM (ed) Med, Kiev, pp 5–104

    Google Scholar 

  • Stojanov N (1972) Astragalus glycyphyllos. In: Ularova K (ed) Our medicinal plants. Nauka and Iskustvo, part I, Sofia, pp 165–166

    Google Scholar 

  • Tappe R, Budzikiewicz H, Ionkova I, Alfermann AW (1994) Triterpene glycosides from transformed root cultures of Astragalus mongholicus Bge. Spectroscopy (IOS Press), 12(1):1–8

    CAS  Google Scholar 

  • Trease GE (1983) Pharmacognosy. Bailliere Tindall, London, PP 358–359

    Google Scholar 

  • Turova AD (1974) Astragalus dsyanthus Pall. In: Medvedev B (ed) Medicinal plants in the USSR and their application. Medicina, Moscow, pp 181–183

    Google Scholar 

  • Vanhala L, Hiltunen R, Oksman-Caldentey MK (1995) Virulence of different Agrobacterium strains on hairy root formation of Hyoscyamus muticus. Plant Cell Rep 14:236–240

    Article  CAS  Google Scholar 

  • Verpoorte R, Van der Heijden, Schripsema J (1993) Plant cell biotechnology for the production of alkaloids: present status and prospects, J Nat Prod 56(2):186–207

    Article  CAS  Google Scholar 

  • Zhon QJ (1985) Chinese medicinal herbs in the treatment of viral hepatitis. In: Chang et al. (eds) Advances in Chinese medicinal materials research. World Sci, Singapore, pp 215–219

    Google Scholar 

  • Zhou Y, Hirotani M, Rui H, Furuya T (1995) Two triglycosidic triterapene astragalosides from hairy root cultures of Astragalus membranaceus. Phytochemistry 38:1407–1410

    Article  CAS  Google Scholar 

  • Zorrequieta A, Ugalde R (1986) Formation in Rhizobium and Agrobacterium spp. of 235-kilodalton protein intermediate in β-D-(1–2)-glucan synthesis. J Bacteriol 167(3):947–952

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Ionkova, I. (1999). Genetic Transformation of Astragalus Species. In: Bajaj, Y.P.S. (eds) Transgenic Medicinal Plants. Biotechnology in Agriculture and Forestry, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58439-8_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-58439-8_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63595-3

  • Online ISBN: 978-3-642-58439-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics