Abstract
Steroid saponins, aculeatiside A and aculeatiside B (Fig. 1), were isolated from the root of Solanum aculeatissimum Jacq. (Solanaceae) (Saijo et al. 1983). Natural steroid sapogenin (diosgenin), from tubers of Dioscorea species is used in the commercial production of steroid hormones, female steroid hormones, and steroidal pharmaceuticals (Ikegami 1981; Imada et al. 1981; Tal et al. 1984; Rokem et al. 1985). Since nuatigenin and isonuatigenin obtained by hydrolysis of aculeatiside A and aculeatiside B (Fig. 1) can be transformed into pregdienolone (Tschesche et al. 1964; Imada et al. 1981; Saijo et al. 1983), it is conceivable that S. aculeatissimum may be a more useful source of pregnane derivatives than the Dioscorea species (Imada et al. 1981). This plant also contains these steroid saponins at a high level; however, it had never been cultivated, and the properties and seasonal variations of growth and steroid saponin production, etc. were also not known. Results of an earlier experiment (Ikenaga et al. 1988a) showed that the content of steroid saponins per dry weight, aculeatiside A and B, increased as the plant grew, reached a peak in November, and decreased slightly in December when the aerial parts of the plant died. The yield of steroid saponins per plant increased in accordance with the rise in root dry weight until November, and then decreased by slow degrees (Table 1).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Bouchez D, Camilleri C (1990) Identification of a putative rol B gene on the TR-DNA of the Agrobacterium rhizogenes A4 Ri plasmid. Plant Mol Biol 14:617–619
Cardi T, Iannamico V, D’Ambrosio F, Filippone E, Lurquin F (1992) Agrobacterium-mediated genetic transformation of Solanum commersonii Dun. Plant Sci 87:179–189
Drewes FE, Staden J van (1995) Initiation of and solasodine production in hairy root cultures of Solanum mauritianum Scop. Plant Growth Regul 17:27–31
Flores HE, Hoy MW, Pickard JJ (1987) Secondary metabolites from root cultures. TIBTECH 5:64–69
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Ikegami N (1981) Steroid production in industry. J Syn Org Chem 39:433
Ikenaga T, Kikuta S, Itimura K, Nakashima K, Matubara T (1988a) Growth and production of steroid saponin in Solanum aculeatissimum during one vegetation period. Planta Med 140–142
Ikenaga T, Kikuta S, Nakashima K (1988b) High-performance liquid chromatographic determination of steroidal sapnin, aculeatiside A and B in Solanum aculeatissimum. JACQ. Chem Pharm Bull 36:416–418
Ikenaga T, Kikuta S, Kistuki M, Yamada M, Nakashima K (1990) Production of steroid saponin in grafts: Solanum aculeatissimum and Lycopersicon esculentum. Hort Science 25:1657–1658
Ikenaga T, Oyama T, Muranaka T (1995) Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum. Plant Cell Rep 14:418–422
Imada Y, Ishikawa H, Nishikawa D (1981) Development of new steroid fermentation. Nippon Nogeikagaku Kaishi 55:713–721
Linsmaier EM, Skoog F (1965) Organic growth factor requirements for tobacco tissue culture. Physiol Plant 18:100–127
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497
Rokem JS, Tal B, Goldberg I (1985) Methods for increasing diosgenin production by Dioscorea cells in suspension cultures. J Nat Prod 48:210–222
Saijo R, Fuke C, Murakami K, Nohara T, Tomimastu T (1983) Two steroidal glycosides, aculeatiside A and B from Solanum aculeatissimum. Phytochemistry 22:733–736
Sauerwein M, Yamazaki T, Shimomura K (1991) Hernandulcin in hairy root cultures of Lippia dulcis. Plant Cell Rep 9:579–581
Signs MW, Flores HE (1990) The biosynthesis potential of plant roots. Bio Essays 12:7–13
Slightom JL, Durand-Tardif M, Jouanin L, Tepfer D (1986) Nucleotide sequence analysis of TL- DNA of Agrobacterium rhizogenes agropine type 7 plasmid: identification of open reading frames. J Biol Chem 261:108–121
Subroto MA, Droran PM (1994) Production of steroidal alkaloids by hairy roots of Solanum aviculare and the effect of gibberellic acid. Plant Cell Tissue Organ Cult 38:93–102
Tal B, Rokem JS, Gressel J, Goldberg I (1984) The effect of chlorophyll-bleaching herbicides on growth, cartenoid and diosgenin levels in cell suspension cultures of Digitalis deltoidea. Phytochemistry 23:1333–1335
Tschesche R, Richert KH (1964) Über Saponine der Spirostanoleihe-xi Nuatigenin, ein Cholegenin-Analogon des Pflanzenreiches. Tetrahedron 20:387–398
White FF, Sinkar VP (1987) Molecular analysis of root induction by Agrobacterium rhizogenes. In: Hohn T, Schell J (eds) Plant DNA infection agents. Springer, Berlin Heidelberg New York, pp 140–177
Yoshimatsu K, Satake M, Shimomura K, Sawada J, Terao T (1990) Determination of cardenolides in hairy root cultures of Digitalis lantan by enzyme-linked immunosorbent assay. J Nat Prod 53:1498–1502
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Ikenaga, T., Muranaka, T. (1999). Genetic Transformation of Solanum aculeatissimum . In: Bajaj, Y.P.S. (eds) Transgenic Medicinal Plants. Biotechnology in Agriculture and Forestry, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58439-8_22
Download citation
DOI: https://doi.org/10.1007/978-3-642-58439-8_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63595-3
Online ISBN: 978-3-642-58439-8
eBook Packages: Springer Book Archive