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Chrysanthemum cinerariaefolium (Pyrethrum): In Vitro Culture and the Production of Pyrethrins and Other Secondary Metabolites

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Medicinal and Aromatic Plants VI

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 26))

Abstract

Chrysanthemum cinerariaefolium (Trev) Boccone is a member of the family Compositae. The genus Chrysanthemum includes over 200 species and is classified as a member of the Anthemidea tribe in the Tubiflorae subdivision of the family Lawrence 1951. Economically, the Compositae family is of considerable importance, containing members important as sources of food, ornamentals and the contact insecticide pyrethrum. The term “pyrethrum flower” often refers to the perennial Chrysanthemum cinerariaefolium (Trev) Bocc., and occasionally to C. coccineum Willd. (garden pyrethrum) and C. marshalli Aschers Lawrence 1951; Staba and Zito 1985.

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References

  • Abou Donia S, Doherty C, Pattenden G (1973) The biosynthesis of chrysanthemum dicarboxylic acid and the origin of the “pyrethrin II’s”. Tetrahedron Lett 36:3477–3480

    Article  Google Scholar 

  • Aoki S, Kaneto K, Hashimoto S, Oogai H (1976) Production of pyrethrins by tissue culture. Jpn Appl Sho 51:97868 (Patent 78:24097 March, 1978)

    Google Scholar 

  • Cashyap MM, Jueh JSH, MacKenzie IA, Pattenden G (1978) In vitro synthesis of pyrethrins from tissue cultures of Tanacetum cinerariaefolium. Phytochemistry 17:544–545

    Article  CAS  Google Scholar 

  • Chumsri P, Staba EJ (1975) Pyrethrin content and larvicidal activity of chrysanthemum plants and tissue cultures. Acad Pharm Sci Abstr 5:169–172

    Google Scholar 

  • Crowley MP, Inglis HS, Snarey M, Thain EM (1961) Biosynthesis of pyrethrins. Nature 191:281–282

    Article  PubMed  CAS  Google Scholar 

  • Crowley MP, Godin PJ, Inglis HS, Snarey M, Thain EM (1962) The biosynthesis of the “pyrethrins.” I. The incorporation of 14C-labelled compounds into the flowers of Chrysanthemum cinerariaefolium and the biosynthesis of chrysanthemum monocarboxylic acid. Biochim Biophys Acta 60:312–319

    CAS  Google Scholar 

  • Davies, JH (1985) The pyrethroids: an historical introduction. In: Leahey JP (ed) The pyrethrum insecticides. Taylor and Francis, London, pp 1–41

    Google Scholar 

  • Doskotch RW, El-Faraly F, Hufford C (1971) Sesquiterpene lactones from pyrethrin flowers. Can J Chem 49:2103–2105

    Article  CAS  Google Scholar 

  • Glennie CW, Harborne JB (1972) Flavonoids of pyrethrum. Pyrethrum Post 11(3): 82–84

    CAS  Google Scholar 

  • Godin PJ, Inglis HS, Snarey M, Thain EM (1963) Biosynthesis of the pyrethrins II. Pyrethric acid and the origin of the ester-methyl group. J Chem Soc (B): 5878–5880

    Google Scholar 

  • Grewal S, Sharma K (1978) Clonal multiplication of medicinal plants by tissue culture. Part IV. Pyrethrum plant (Chrysanthemum cinerariaefolium Vis.) regeneration from shoot tip culture. Indian J Exp Biol 16:1119

    CAS  Google Scholar 

  • Head SW (1966) A study of the insecticidal constituents in Chrysanthemum cinerariaefolium. Pyrethrum Post 8:32–37

    CAS  Google Scholar 

  • Head SW (1968) Fatty acid composition of the extract from pyrethrum flowers (Chrysanthemum cinerariaefolium). J Agric Food Chem 16(5): 762–765

    Article  CAS  Google Scholar 

  • Head SW (1970) World standard pyrethrum extract. Pyrethrum Post 10(3): 27–31

    CAS  Google Scholar 

  • Head SW (1973) Composition of pyrethrum extract and analysis. In: Casida JE (ed) Pyrethrum, the natural insecticide. Academic Press, New York, pp 25–48

    Google Scholar 

  • Hitchcock C (1975) Structure and distribution of plant acyl lipids. In: Galliard T, Mercer EI (eds) Recent advances in the chemistry and biochemistry of plant lipids. Academic Press, London, pp 1–18

    Google Scholar 

  • Jones GDG (1973) Pyrethrum production. In: Casida JE (ed) Pyrethrum, the natural insecticide. Academic Press, New York, pp 17–22

    Google Scholar 

  • Karki A, Rajbhandary SB (1984) Clonal propagation of Chrysanthemum cinerariaefolium Vis. (pyrethrum) through tissue culture. Pyrethrum Post 15(4): 118–121

    Google Scholar 

  • Kueh JS, MacKenzie IA, Pattenden G (1985) Production of chrysanthemic acid and pyrethrins by tissue cultures of Chrysanthemum cinerariaefolium. Plant Cell Rep 4:118

    Article  CAS  Google Scholar 

  • Lawrence G (1951) Taxonomy of vascular plants. Macmillan, New York

    Google Scholar 

  • Levy LW (1981) A large-scale application of tissue culture: the mass propagation of pyrethrum clones in Ecuador. Environ Exp Bot 21:389–395

    Article  Google Scholar 

  • McLaughlin GA (1973) History of pyrethrum. In: Casida JE (ed) Pyrethrum, the natural insecticide. Academic Press, New York, pp 3–13

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Pattenden G (1970) Some studies on the biosynthesis of the pyrethrins. Pyrethrum Post 10(4): 2–5

    CAS  Google Scholar 

  • Pattenden G, Storer R (1973) Studies on the biosynthesis of chrysanthemum monocarboxylic acid. Tetrahedron Lett 36:3474–3476

    Google Scholar 

  • Porter JN, Sathyamoorthy N, Spurgeon SL (1984) Isopentenyl pyrophosphate isomerase and prenyltransferase from tomato fruit plastids. Arch Biochem Biophys 230:446–448

    Article  PubMed  Google Scholar 

  • Roest S (1976) Flowering and vegetative propagation of pyrethrum (Chrysanthemum cinerariaefolium Vis) in vivo and in vitro. Centre Agric Pub and Doc, Wageningen, pp 104–109

    Google Scholar 

  • Roest S, Bokelmann GS (1973) Vegetative propagation of Chrysanthemum cinerariaefolium in vitro. Sci Hortic 1:120–122

    Article  Google Scholar 

  • Satterwhites DM (ed) (1985) Isopentenyldiphosphate Delta-Isomerase, Methods Enzymol 110:92–99

    Google Scholar 

  • Staba EJ, Zito SW (1985) The production of pyrethrins by Chrysanthemum cinerariaefolium (Trev) Boccone. In: Neumann A, Barz W, Reinhard E, (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York, pp 209–214

    Chapter  Google Scholar 

  • Staba EJ, Nygaard BG, Zito SW (1984) Light effects on pyrethrum shoot cultures. Plant Cell Tissue Organ Cult 3:211–214

    Article  CAS  Google Scholar 

  • Stevenson DS (1972) Application of liquid-gel chromatography to the analytical characterization of pyrethrum extract. Pyrethrum Post 11(3): 90–93

    CAS  Google Scholar 

  • Tio C (1989) Comparative studies of fatty acids, monoterpenes and pyrethrins production in pyrethrum [Chrysanthemum cinerariaefolium (Trev.) Bocc.] leaves, plantlets and callus tissue cultures. PhD Dissertation, St. John’s University College of Pharmacy and Allied Health Professions, Jamaica Queens New York

    Google Scholar 

  • Wambugu FM, Rangan TS (1981) In vitro clonal multiplication of pyrethrum (Chrysanthemum cinerariaefolium Vis) by micropropagation. Plant Sci Lett 22(3): 219–226

    Article  CAS  Google Scholar 

  • Zieg RG, Zito SW, Staba EJ (1983) Selection of high pyrethrin-producing tissue cultures. Planta Med 48:88–91

    Article  PubMed  CAS  Google Scholar 

  • Zito SW, Tio CD (1990) Constituents of Chrysanthemum cinerariaefolium in leaves, regenerated plantlets and callus. Phytochemistry 29(8): 2533–2534

    Article  CAS  Google Scholar 

  • Zito SW, Tio CD (1992) Comparison of fatty acids in the pyrethrum extract from Chrysanthemum cinerariaefolium flowers, leaves, regenerated plantlets and callus. J Pharm Sci (submitted)

    Google Scholar 

  • Zito SW, Zieg RG, Staba EJ (1983) Distribution of pyrethrins in oil glands and leaf tissue of Chrysanthemum cinerariaefolium. Planta Med 47:205–207

    Article  PubMed  CAS  Google Scholar 

  • Zito SW, Srivastava V, Adebayo-Olojo E (1991) Incorporation of [1-14C]-isopentenyl pyrophosphate into monoterpenes by a cell-free homogenate prepared from callus cultures of Chrysanthemum cinerariaefolium. Planta Med 57:425–427

    Article  PubMed  CAS  Google Scholar 

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© 1994 Springer-Verlag Berlin Heidelberg

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Zito, S.W. (1994). Chrysanthemum cinerariaefolium (Pyrethrum): In Vitro Culture and the Production of Pyrethrins and Other Secondary Metabolites. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants VI. Biotechnology in Agriculture and Forestry, vol 26. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57970-7_4

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  • DOI: https://doi.org/10.1007/978-3-642-57970-7_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63420-8

  • Online ISBN: 978-3-642-57970-7

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