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Transformation of Maize Protoplasts

  • Chapter
Maize

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 25))

Abstract

The early 1980s were marked by the first reports (subsequently confirmed and reproduced in other labs) on Ti plasmid DNA transformation of plant protoplasts (Davey et al. 1980; Krens et al. 1982). Soon after, however, research activities were directed toward the genetic transformation of plant cells, rather than protoplasts, using Agrobacterium tumefaciens as a natural DNA transfer system (review: Hooykaas 1989). The genetic transformation of dicotyledonous plants was achieved but the Agrobacterium system proved to be ineffective in transformation of most monocot species. Attempts to show Agrobacterium infection of maize cells met with only partial success (Graves and Goldman 1986; Grimsley et al. 1987).

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, 47907, USA

    L. A. Lyznik & T. K. Hodges

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  1. L. A. Lyznik
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  2. T. K. Hodges
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  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1994 Springer-Verlag Berlin Heidelberg

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Lyznik, L.A., Hodges, T.K. (1994). Transformation of Maize Protoplasts. In: Bajaj, Y.P.S. (eds) Maize. Biotechnology in Agriculture and Forestry, vol 25. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57968-4_15

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  • DOI: https://doi.org/10.1007/978-3-642-57968-4_15

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  • Print ISBN: 978-3-642-63419-2

  • Online ISBN: 978-3-642-57968-4

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