Abstract
Crop improvement in cultivated tobacco (Nicotiana tabacum L.) has a long history of utilization of wild germplasm as a source of disease resistance genes. Genes for resistance have been obtained from several wild Nicotiana species and N. tabacum accessions. These include tobacco mosaic virus (TMV) and powdery mildew resistance from N. glutinosa, wildfire and black shank resistance from N. longiflora, black shank resistance from N. plumbaginifolia, black root rot and blue mold resistance from N. debneyi, blue mold resistance from N. goodspeedii and N. velutina, root knot nematode resistance from N. tomentosa and N. megalosiphon, fusarium wilt and bacterial wilt resistance from the N. tabacum accession 448A, and black root rot, black shank, powdery mildew, and root knot nematode resistance from other N. tabacum accessions (Burk and Heggestad 1966; Lucas 1975; Stavely 1979). One wild species which has not been used successfully as a source of disease-resistance genes is N. repanda (Fig. 1). N. repanda has been reported to be resistant to more tobacco diseases and pathogens than any other Nicotiana species (Table 1). To date, no cultivars are available carrying disease-resistance genes derived from this species. Two species of root knot nematodes, Meloidogyne arenaria and M. javanica, are an increasingly prevalent problem on tobacco in the southeastern United States, and N. repanda has a high level of resistance to both of them. Current cultivars lack resistance to these nematode species, and the development of cultivars containing resistance genes from N. repanda would be highly desirable.
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References
Bates GW (1990) Asymmetric hybridization between Nicotiana tabacum and N. repanda by donor protoplast fusion: transfer of TMV resistance. Theor Appl Genet 80: 481–487
Bui PT, Jenns AE, Schneider SM, Daub ME (1992) Resistance to tobacco mosaic virus and Meloidogyne arenaria in fusion hybrids between Nicotiana tabacum and an N. repanda × N. sylvestris hybrid. Phytopathology 82: 1305–1310
Burk LG (1967) An interspecific bridge-cross. J Hered 58: 215–218
Burk LG, Heggestad HE (1966) The genus Nicotiana: a source of resistance to diseases of cultivated tobacco. Econ Bot 20: 76–88
Davis EL, Rich JR, Gwynn GR (1988a) Reaction of selected Nicotiana spp. × N. tabacum crosses grown in microplots to three Meloidogyne spp. Nematropica 18: 109–115
Davis EL, Rich JR, Gwynn GR, Sisson V (1988b) Greenhouse evaluation of Nicotiana spp. for resistance to root-knot nematodes. Nematropica 18: 99–107
DeVerna JW, Myers JR, Collins GB (1987) Bypassing prefertilization barriers to hybridization in Nicotiana using in vitro pollination and fertilization. Theor Appl Genet 73: 665–671
Evans DA (1981) Disease resistance: incorporation into sexually incompatible somatic hybrids of the genus Nicotiana. Science 213: 907–909
Foster HH (1943) Resistance in the genus Nicotiana to Phytophthora parasitica Dastur var. nicotiana Tucker. Phytopathology 33: 403–404
Gwynn GR, Barker KR, Reilly JJ, Komn DA, Burk LG, Reed SM (1986) Genetic resistance to tobacco mosaic virus, cyst nematodes, root-knot nematodes and wildfire from Nicotiana repanda incorporated into N. tabacum. Plant Dis 70: 958–962
Iwai S, Kishi C, Nakata K, Kubo S (1985) Production of a hybrid of Nicotiana repanda Willd. × N. tabacum L. by ovule culture. Plant Sci 41: 175–178
Jenns AE, Daub ME, Reed SM (1988) Limitation of the Su albino gene marker system in production of fusion hybrids between highly incompatible Nicotiana species. Tob Sci 32: 77–81
Kincaid RR (1949) Three interspecific hybrids of tobacco. Phytopathology 39: 284–287
Kubo T (1988) Protoplast fusion in tobacco breeding. In: Cell and tissue culture in field crop improvement. Iwata Experimental Station. Japan Tobacco, Iwata pp 49–53
Lea HW, Willetts HJ (1963) The development of a nematode-resistant tobacco variety for Australian conditions, II. In: The transference of resistance from Nicotiana repanda to N. tabacum. Proc Third World Tob Sci Congr, pp 296–298
Lloyd R (1975) Tissue culture as a means of circumventing lethality in an interspecific Nicotiana hybrid. Tob Sci 19:4–6
Lucas GB (1975) Diseases of tobacco, 3rd edn. Biological Consulting Associates, Raleigh, N
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Nagata T, Takebe I (1971) Plating of isolated mesophyll protoplasts on agar medium. Planta 99: 12–20
Pittarelli GW Stavely JR (1975) Direct hybridization of Nicotiana repanda × N. tabacum. J Hered 66: 281–284
Reed SM, Collins GB (1978) Interspecific hybrids in Nicotiana through in vitro culture of fertilized ovules. J Hered 69: 311–315
Rothstein SJ, Lahners DN, Lotstein RJ, Carozzi NB, Jayne SM, Rice DA (1987) Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation. Gene 53: 153–161
Shintaku Y, Yamamoto K, Nakajima T (1988) Interspecific hybridization between Nicotiana repanda Willd. and N. tabacum L. through the pollen irradiation technique and the egg irradiation technique. Theor Appl Genet 76: 293–298
Stavely JR (1979) Disease resistance. In: Durbin RD (ed) Nicotiana: procedures for experimental use. US Dep Agric Tech Bull 1586: 87–110
Stavely JR, Pittarelli GW, Burk GL (1973) Nicotiana repanda as a potential source for disease resistance in N. tabacum. J Hered 64: 265–271
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© 1994 Springer-Verlag Berlin Heidelberg
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Daub, M.E., Bui, P.T., Jenns, A.E. (1994). Somatic Hybrids Between Nicotiana repanda and N. tabacum Show Resistance to Tobacco Mosaic Virus and Meloidogyne arenaria . In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_25
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DOI: https://doi.org/10.1007/978-3-642-57945-5_25
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