Abstract
To be able to perform successful somatic hybridization, a stringent system is required to select for and allow the propagation of true somatic hybrids but eliminate the growth of unfused partners and fusion products formed from protoplasts of the same type.
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References
Bajaj YPS (1977) Protoplast isolation, culture, and somatic hybridization. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Berlin Heidelberg New York, pp 467–496
Bajaj YPS (1988) Regeneration of plants from frozen (−196 °C) protoplasts of Atropa belladonna L., Datura innoxia Mill, and Nicotiana tabacum L. Indian J Exp Biol 26: 289–292
Caboche M (1980) Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium. Planta 149: 7–18
Du XD, Mourad GS, Williams D, King J (1991) Somatic hybrids derived from fusion of a universal hybridizer and a wild-type line of Datura. Plant Sci 79: 111–118
Furner IJ, King J, Gamborg OL (1978) Plant regeneration from protoplasts isolated from a predominantly haploid suspension culture of Datura innoxia (Mill.). Plant Sci Lett 11: 169–176
Gleba YY, Hoffmann F (1980) Arabidobrassica: a novel plant obtained by protoplast fusion. Planta 149: 112–117
Glimelius K, Eriksson T, Grafe R, Müller AJ (1978) Somatic hybridization of nitrate reductase-deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol Plant 44: 273–277
Hamill JD, Pental D, Cocking EC, Müller AJ (1983) Production of nitrate reductase deficient streptomycin-resistant mutant of Nicotiana tabacum for somatic hybridization studies. Heredity 50: 197–200
Harms CT (1983) Somatic hybridization by plant protoplast fusion. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983. Proc 6th Int Protoplast Symp. Birkhäuser, Basel, pp 69–84
Horsch RB, King J (1984) The isolation of auxotrophs from Datura innoxia Mill. cell cultures following recovery of arsenate-treated cells on feeder plates. Planta 160: 168–173
Horsch RB, King J, Jones GE (1980) Measurement of cultured plant cell growth on filter paper discs. Can J Bot 28: 2402–2406
Kao KN (1986) Fusion of plant protoplasts at the interface of a glucose-and a sucrose-polyethylene glycol solution. J Plant Physiol 126: 55–58
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110
King J (1986) Plant cells and the isolation of conditional lethal variants. Enzyme Microb Technol 9: 514–522
Krumbiegel G, Schieder O (1981) Comparison of somatic and sexual incompatibility between Datura innoxia and Atropa belladonna. Planta 153: 466–470
Larkin PJ (1976) Purification and viability determination of plant protoplasts. Planta 128: 213–216
Maliga P, Lazar G, Nagy AH, Menczel L (1978) Restoration of morphogenetic potential in Nicotiana by somatic hybridization. Mol Gen Genet 157: 291–296
McDonnell RE, Clark RD, Smith WA, Hinchee MA (1987) A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissues. Plant Mol Biol Rep 5: 380–386
Mii M, Seeni S, Fowke LC, King J (1985) The isolation and cultivation of protoplasts from cell suspensions of a pantothenate-requiring auxotroph of Datura. Can J Bot 63: 779–783
Müller AG, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161: 67–76
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473–497
Savage AD, King J, Gamborg OL (1979) Recovery of a pantothenate auxotroph from cell suspension culture of Datura innoxia Mill. Plant Sci Lett 16: 367–376
Saxena PK, King J (1987a) Complementation by intraspecific protoplast fusion of auxotrophic cell lines of Datura innoxia P. Mill. Plant Cell Tissue Organ Cult 9: 61–71
Saxena PK, King J (1987b) Optimal conditions for the isolation and culture of protoplasts from a cell suspension culture of an isoleucine-valine-requiring auxotroph of Datura innoxia. Can J Bot 65: 1736–1740
Saxena PK, Mii M, Crosby WL, Fowke LC, King J (1986a) Transplantation of isolated nuclei into plant protoplasts: a novel technique for introducing foreign DNA into plant cells. Planta 168: 29–35
Saxena PK, Mii M, King J (1986b) Genetic complementation by somatic hybridization between pantothenate-and adenine-requiring auxotrophs of Datura innoxia P. Mill. Can J Genet Cytol 28: 1022–1025
Saxena PK, Hammerlindl J, Crosby WL, King J (1990) Introduction of resistance to kanamycin into the protoplasts from a pantothenate-requiring auxotroph of Datura innoxia P. Mill. via direct gene transfer. Plant Sci 70: 105–114
Schieder O (1975) Selection of a somatic hybrid between auxotrophic mutants of Sphaerocarpos donnellii using the method of protoplast fusion. Z Pflanzenphysiol 74: 357–365
Schieder O (1980) Somatic hybrids between a herbaceous and two tree Datura species. Z Pflanzenphysiol 98: 119–127
Toriyama K, Kameya T, Hinata K (1987) Selection of a universal hybridizer in Sinapis turgida Del and regeneration of plantlets from somatic hybrids with Brassica species. Planta 170: 308–313
Wallsgrove RM, Risiott R, King J, Bright SWJ (1986) Biochemical characterization of an auxotroph of Datura innoxia requiring isoleucine and valine. Plant Sci 43: 109–114
Ye J, Hauptmann RM, Smith AG, Widholm JM (1987) Selection of a Nicotiana plumbaginifolia universal hybridizer and its use in intergeneric somatic hybrid formation. Mol Gen Genet 208: 474–480
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© 1994 Springer-Verlag Berlin Heidelberg
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Mourad, G., King, J. (1994). Somatic Hybridization in Datura . In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_24
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DOI: https://doi.org/10.1007/978-3-642-57945-5_24
Publisher Name: Springer, Berlin, Heidelberg
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