Skip to main content

Somatic Hybridization Between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun

  • Chapter
Somatic Hybridization in Crop Improvement I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 27))

  • 292 Accesses

Abstract

For Lycopersicon esculentum, symmetric intergeneric and interspecific somatic hybrids were regenerated following fusion with protoplasts of Solanum tuberosum (Melchers et al. 1978 ; Shepard et al. 1983), S. lycopersicoides (Handley et al. 1986; Tan 1987; Guri et al. 1991), S. rickii (O’Connell and Hanson 1986), S. nigrum (Guri et al. 1988), S. acaule (Schweizer et al. 1988), S. muricatum (Sakomoto and Taguchi 1991), L. chilense (Bonnema and O’Connell 1992), L. pennellii (O’Connell and Hanson 1987), L. peruvianum (Kinsara et al. 1986; Wijbrandi 1989; San et al. 1990), and L. peruvianum var. dentatum (Piven et al. 1986). Nevertheless, the only work reporting fertility of such hybrids and successful production of sexual F1 progeny is that of (1989). The asymmetric nuclear hybrids for Lycopersicon were produced by fusion of the L. esculentum protoplasts with the γ-irradiated protoplasts of L. pennellii (O’Connell and Hanson 1987; Wachocki et al. 1991), L. peruvianum (Wijbrandi 1989), and with UV-inactivated protoplasts of S. nigrum (Jain et al. 1988). All of these were, however, sterile

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984) Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196: 244–253

    Article  CAS  Google Scholar 

  • Bates GW (1990) Asymmetric hybridization between Nicotiana tabacum and N. repanda by donor recipient protoplast fusion: transfer of TMV resistance. Theor Appl Genet 80: 481–487

    Article  Google Scholar 

  • Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet 74: 718–726

    Article  Google Scholar 

  • Bonnema AB, O’Connell MA (1992) Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) and Lycopersicon chilense. Plant Cell Rep 10: 629–632

    Article  CAS  Google Scholar 

  • Bonnema AB, Melzer JM, O’Connell MA (1991) Tomato cybrids with mitochondrial DNA from Lycopersicon pennellii. Theor Appl Genet 81: 339–348

    Article  Google Scholar 

  • Bookjans G, Stummann BM, Henningsen KW (1984) Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength. Anal Biochem 141: 244–247

    Article  PubMed  CAS  Google Scholar 

  • Brewer GJ (1970) An introduction to isozyme techniques. Academic Press, New York, 230 pp

    Google Scholar 

  • Dudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey V, Cella R (1987) Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: regeneration of fertile plants. Proc Natl Acad Sci USA 84: 8434–8438

    Article  PubMed  CAS  Google Scholar 

  • Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parokonny AS, Borysyuk NV, Cherep NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by “gamma-fusion”. Plant Sci 61: 105–117

    Article  Google Scholar 

  • Famelaer I, Negrutiu I, Mouras A, Vaucheret H, Jacobs M (1990) Asymmetric hybridization in Nicotiana by “gamma-fusion” and progeny analysis of self-fertile hybrids. Theor Appl Genet 79: 513–520

    Article  Google Scholar 

  • Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Borysyuk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”. Theor Appl Genet 76: 760–766

    Article  Google Scholar 

  • Glimelius K, Bonnet HT (1986) Nicotiana cybrids with Petunia chloroplasts. Theor Appl Genet 72: 794–798

    Article  CAS  Google Scholar 

  • Guri A, Levi A, Sink KC (1988) Morphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum. Mol Gen Genet 212: 191–198

    Article  CAS  Google Scholar 

  • Guri A, Dunbar LJ, Sink KC (1991) Somatic hybridization between selected Lycopersicon and Solanum species. Plant Cell Rep 10: 76–80

    Article  Google Scholar 

  • Hagemann R (1982) Induction of plastome mutations by nitroso-urea-compounds. In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 119–126

    Google Scholar 

  • Handley LW, Nickels RL, Cameron MW, Moore PP, Sink KC (1986) Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides. Theor Appl Genet 71: 691–697

    Article  Google Scholar 

  • Hinnisdaels S, Bariller L, Mouras A, Sidorov V, Del-Favero J, Veuskens J, Negrutiu I, Jacobs M (1991) Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma”-fusion. Theor Appl Genet 82: 609–614

    Article  Google Scholar 

  • Hosticka LP, Hanson MR (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J Hered 75: 242–246

    CAS  Google Scholar 

  • Imanishi S, Hiura I (1983) Culture and regeneration of Lycopersicon peruvianum leaf protoplasts. Jpn J Breed 33: 359–368

    Google Scholar 

  • Jain SM, Shahin EA, Sun S (1988) Interspecific protoplast fusion for the transfer of atrazine resistance from Solanum nigrum to tomato (Lycopersicon esculentum L.). Plant Cell Tissue Organ Cult 12: 189–192

    Article  CAS  Google Scholar 

  • Kinsara A, Patnaik SN, Cocking EC, Power JB (1986) Somatic hybrid plants of Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill. J Plant Physiol 125: 225–234

    Article  Google Scholar 

  • Kosova AI, Kyku VN (1986) Cytoembryology of tomato. Shtiintsa, Kishinev, 230 pp

    Google Scholar 

  • McGuire DC, Rick CM (1954) Self-incompatibility in species of Lycopersicon sect. Eriopersicon and hybrids with Lycopersicon esculentum. Hilgardia 23: 101–124

    Google Scholar 

  • Medgyesy P (1990) Selection and analysis of cytoplasmic hybrids. In: Dix PJ (ed) Plant cell line selection. VCH, New York, pp 287–316

    Google Scholar 

  • Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance chloroplast transfer from Nicotiana sylvestris and isolation of their somatic hybrids. Mol Gen Genet 179: 693–698

    Article  CAS  Google Scholar 

  • Medgyesy P, Golling R, Nagy F (1985) A light sensitive recipient for the effective transfer of chloroplast and mitochondrial traits by protoplast fusion in Nicotiana. Mol Gen Genet 70: 590–594

    CAS  Google Scholar 

  • Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43: 203–218

    Article  Google Scholar 

  • Melzer JM, O’Connell MA (1990) Molecular analysis of the extent of asymmetry in two asymmetric somatic hybrids of tomato. Theor Appl Genet 79:193–200

    Article  CAS  Google Scholar 

  • Menczel L, Nagy F, Kiss ZR, Maliga P (1981) Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Theor Appl Genet 59: 191–195

    Article  CAS  Google Scholar 

  • Mühlbach HP (1980) Different regeneration potentials of mesophyll protoplasts from cultivated and a wild species of tomato. Planta 148: 89–96

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Neal CA, Topoleski LD (1983) Effects of basal medium on growth of immature tomato embryos in vitro. J Am Soc Hortic Sci 108:434–438

    CAS  Google Scholar 

  • O’Connell MA, Hanson MR (1986) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii. Theor Appl Genet 72: 59–65

    Google Scholar 

  • O’Connell MA, Hanson MR (1987) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and L. pennellii. Theor Appl Genet 75: 83–89

    Google Scholar 

  • Palmer JD (1986) Isolation and structural analysis of chloroplast DNA. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 118. Academic Press, London, pp 167–186

    Google Scholar 

  • Piven NM, Makhorina OK (1985) Plant regeneration from callus protoplasts of Lycopersicon peruvianum var. dentatum. Sov Cytol Genet 19: 271–277

    CAS  Google Scholar 

  • Piven NM, Makhorina OK (1986) Culture of isolated protoplasts of leaf mesophyll and regeneration of tomato plants and its certain wild relatives. Sov Physiol Biochem Cult 18: 166–171

    CAS  Google Scholar 

  • Piven NM, Makhorina OK, Komarnitsky IK, Cherep NN, Shlumukov LR (1986) Somatic hybridization of Lycopersicon esculentum Mill. and Lycopersicon peruvianum v. dentatum. Sov Biopolymers Cell 2: 136–140

    CAS  Google Scholar 

  • Ratushnyak YI (1991) Transgenomic plants in Lycopersicon genus obtained by “gamma-hybridization” of protoplasts. PhD Thesis, Institute of Genetics and Cytology, Minsk p 16

    Google Scholar 

  • Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybridization of tomatoes by “gamma-fusion” of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Plant Sci 73: 65–78

    Article  Google Scholar 

  • Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993a) Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. Mol Gen Genet 236: 427–432

    Article  PubMed  CAS  Google Scholar 

  • Ratushnyak YI, Cherep NN, Zavgorodnyaya AV, Latypov SA, Borozenko IV, Rachkovskaya RI, Gleba YY (1993b) Cell engineering synthesis of tomato fertile asymmetric somatic hybrids by “γ-fusion” of protoplasts. Russ Genet 29: 154–166

    Google Scholar 

  • Rick CM (1983) Tomato (Lycopersicon). In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding. Elsevier, Amsterdam, pp 147–165

    Google Scholar 

  • Rick CM, Lamm R (1955) Biosystematic studies on the status of Lycopersicon chilense. Am J Bot 42: 663–675

    Article  Google Scholar 

  • Sakomoto K, Taguchi T (1991) Regeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum. Theor Appl Genet 81: 509–513

    Article  Google Scholar 

  • Samsonova IA (1970) Investigations on the mutability of the plastome. I Characterization of a plastome mutant of tomatoes. Sov Genet 6: 36–41

    Google Scholar 

  • San LH, Vedel F, Sihachakr D, Remy R (1990) Morphological and molecular characterization of fertile tetraploid somatic hybrids produced by protoplast electrofusion and PEG-induced fusion between Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill. Mol Gen Genet 221: 17–26

    Article  CAS  Google Scholar 

  • Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor Appl Genet 75: 679–684

    Article  CAS  Google Scholar 

  • Shahin EA (1985) Totipotency of tomato protoplasts. Theor Appl Genet 69: 235–240

    Article  CAS  Google Scholar 

  • Shepard JF, Bidney D, Barsby T, Kemble A (1983) Genetic transfer in plants through interspecific protoplast fusion. Science 219: 683–688

    Article  PubMed  CAS  Google Scholar 

  • Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152: 341–345

    Article  CAS  Google Scholar 

  • Somers DA, Narayanan KR, Kleinhofs A, Cooper-Bland S, Cocking EC (1986) Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion. Mol Gen Genet 204: 296–301

    Article  CAS  Google Scholar 

  • Tan MMC (1987) Somatic hybridization and cybridization in some Solanaceae. PhD Thesis, Free University, Amsterdam, 111 pp

    Google Scholar 

  • Wachocki SE, Bonnema AB, O’Connell MA (1991) Comparison of the organization of the mitochondrial genome in tomato somatic hybrids and cybrids. Theor Appl Genet 81: 420–427

    Article  Google Scholar 

  • Wijbrandi J (1989) Isolation and characterisation of somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum. PhD Thesis, Agricultural University, Wageningen, 104 pp

    Google Scholar 

  • Wilson AJ, Chourey PS (1984) A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources. Plant Cell Rep 3: 237–239

    Article  CAS  Google Scholar 

  • Zapata FJ, Sink KC (1981) Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts. Theor Appl Genet 59: 265–268

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Ratushnyak, Y.I., Cherep, N.N., Latypov, S.A. (1994). Somatic Hybridization Between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-57945-5_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63411-6

  • Online ISBN: 978-3-642-57945-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics