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Regeneration of Plants from Protoplasts of Capsella bursa-pastoris (L.) Medic. (Shepherd’s Purse)

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Plant Protoplasts and Genetic Engineering VI

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 34))

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Abstract

Shepherd’s purse [Capsella bursa-pastoris (L.) Medic. x = 8; 2n = 32] is a wild cruciferous species originating from the Mediterranean lands, that is often found on wastelands and on cultivated fields throughout the world. It is resistant to Alternaria brassicae (Tewari 1991), is cold-tolerant, has a short life cycle, and most importantly, it is unattractive to flea beetles (Feeny et al. 1970). Flea beetles (Phyllotreta cruciferae and P. striolata) inflict heavy losses in cruciferous crops on the Canadian Prairies. These insects overwinter, emerge early in spring to attack seedlings of most wild and cultivated species of the Brassicaceae during May and June, feed on the cotyledons and first true leaves, and continue feeding on mature plants and seed pods as they ripen (Lamb 1984).

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References

  • Bonfils A.-C, Gleddie SC, Webb JA, Keller WA (1992) Somatic embryogenesis from cell suspension and protoplast cultures of Capsella bursa-pastoris (L.) Medic. In Vitro Cell Dev Biol 28P: 137–142

    Google Scholar 

  • Coleman AW, Goff LJ (1985) Applications of fluorochromes to pollen biology. I. Mithramycin and 4’, 6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA. Stain Technol 60: 145–154

    CAS  Google Scholar 

  • Douglas GC, Keller WA, Setterfield G (1981) Somatic hybridization between Nicotiana rustica and N. tabacum. I. Isolation and culture of protoplasts and regeneration of plants from cell cultures of wild-type and chlorophyll-deficient strains. Can J Bot 59: 208–219

    CAS  Google Scholar 

  • Earle ED (1994) Analysis of somatic hybrids and cybrids obtained by fusion of Brassica rapa and B. oleracea. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 27. Somatic hybridization in crop improvement I. Springer, Berlin Heidelberg New York, pp 305–319

    Google Scholar 

  • Feeny P, Paauwe KL, Demong NJ (1970) Flea beetles and mustard oils: host plant specificity of Phyllotetra cruciferae and P. striolata adults (Coleoptera: Chrysomelidae). Ann Entomol Soc Am 63: 832–841

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Gleddie SC (1989) Plant regeneration from cell suspension cultures of Arabidopsis thaliana heynh. Plant Cell Rep 8: 1–5

    Article  Google Scholar 

  • Glimelius K (1984) High growth rate and regeneration capacity of hypocotyl protoplasts in some Brassicaceae. Physiol Plant 61: 38–44

    Article  CAS  Google Scholar 

  • Glimelius K, Fahleson J, Landgren M, Sjödin C, Sundberg E (1991) Gene transfer via somatic hybridization in plants. Trends Biotechnol 9: 24–30

    Article  Google Scholar 

  • Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367

    Article  CAS  Google Scholar 

  • Kao HM, Séguin-Swartz G (1987) Study of factors affecting the culture of Brassica napus L. and B. juncea Coss. mesophyll protoplasts. Plant Cell Tissue Organ Cult 10: 79–90

    Article  CAS  Google Scholar 

  • Klimaszewska K, Keller WA (1985) Somatic embryogenesis in cell suspension and protoplast cultures of Brassica nigra (L.) Koch. J Plant Physiol 122: 251–260

    Article  Google Scholar 

  • Lamb RJ (1984) Effects of flea beetles, Phyllotreta spp. (Chrysomelidae: Coleoptera), on the survival, growth, seed yield and quality of canola, rape and yellow mustard. Can Entomol 116: 269–280

    Google Scholar 

  • Lichter R (1981) Anther culture of Brassica napus in a liquid culture medium. Z Pflanzenphysiol 103: 229–237

    CAS  Google Scholar 

  • Loh C-S, Ingram DS, Hanke DE (1983) Cytokinins and the regeneration of plantlets from secondary embryoids of winter oilseed rape, Brassica napus ssp. oleifera. New Phytol 95: 349–358

    Article  CAS  Google Scholar 

  • Monnier M (1978) Culture of zygotic embryos. In: Thorpe TA (ed) Frontiers of plant tissue culture, 1978. The International Association for Plant Tissue Culture, University of Calgary, Printing Press, Calgary, pp 277–286

    Google Scholar 

  • Monnier M (1984) Survival of young immature Capsella embryos cultured in vitro J Plant Physiol 115: 105–113

    CAS  Google Scholar 

  • Monnier M, Lagriffol J (1986) Effect of ovular tissue on the development of Capsella embryos cultivated in vitro. J Plant Physiol 122: 17–24

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Pelletier G, Primard C, Vedel F, Chétrit P, Rémy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191: 244–250

    Article  CAS  Google Scholar 

  • Tewari JP (1991) Current understanding of resistance to Alternaria brassicae in Crucifers. In: Rapeseed in a changing world. GCIRC 8th Int Rapeseed Congr, July 9–11, 1991, Saskatoon, Saskatchewan, p 84

    Google Scholar 

  • Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47: 189–194

    PubMed  CAS  Google Scholar 

  • Zilkah S, Gressel J (1977) Cell cultures vs whole plants for measuring phytotoxicity. I. The establishment and growth of callus and suspension cultures; definition of factors affecting toxicity on calli. Plant Cell Physiol 18: 641–655

    CAS  Google Scholar 

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© 1995 Springer-Verlag Berlin Heidelberg

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Bonfils, AC., Gleddie, S.C., Keller, W.A. (1995). Regeneration of Plants from Protoplasts of Capsella bursa-pastoris (L.) Medic. (Shepherd’s Purse). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VI. Biotechnology in Agriculture and Forestry, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57840-3_3

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  • DOI: https://doi.org/10.1007/978-3-642-57840-3_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63374-4

  • Online ISBN: 978-3-642-57840-3

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