Skip to main content
SpringerLink
Log in

Genetic Transformation in Dendrobium (Orchid)

  • Chapter
Plant Protoplasts and Genetic Engineering VI

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 34))

Abstract

Dendrobium is one of the largest genera, in the family Orchidaceae with an estimated 1400 species (Dressler 1990). It is found throughout western Asia and the Pacific Islands. Subgeneric sections of Dendrobium species with horticultural value include Phalaenanthe, Spatulata (Ceratobium), Latourea, Dendrobium (Eugenanthe), Callista, and Formosae (Nigrohirsutae). Intersectional polyploid hybrids comprise the majority of commercial cut flower and potted cultivars (Kamemoto 1987). Exotic flowers on long arching sprays offer great diversity in flower form and color, and a long vase life of 2 to 3 weeks. Since the 1960s, dendrobiums have become a leading crop among Pacific Rim flower growers due to their relative ease of culture, postharvest handling, and the increased availability of cultivars. In 1991, Thailand exported about 300 million orchid sprays (mainly dendrobiums) valued at FOB $26.2 million. In Hawaii, in 1992 Dendrobium orchid sales reached a farmgate value of $7.7 million for cut sprays, potted plants and lei flowers (Hawaii Agricultural Statistics Service 1993). The global market for quality dendrobiums continues to be strong.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abdul-Baki AA, Saunders JA, Matthews BF, Pittarelli GW (1990) DNA uptake during electroporation of germinating pollen grains. Plant Sci 70: 181–190

    Article  CAS  Google Scholar 

  • Ahokas H (1989) Transfection of germinating barley seed electrophoretically with exogenous DNA. Theor Appl Genet 77: 469–472

    Article  CAS  Google Scholar 

  • Arditti J, Ernst R (1992) Micropropagation of orchids. John Wiley, New York, pp 311–365

    Google Scholar 

  • Chee PP, Fober KA, Slightom JL (1989) Transformation of soybean (Glycine max) by infecting germinating seeds with Agrobacterium tumefaciens. Plant Physiol 91: 1212–1218

    Article  PubMed  CAS  Google Scholar 

  • Chia TF, Chan YS, Chua NH (1990) Genetic engineering of tolerance to Cymbidium Mosaic Virus. In: Kernohan J, Bonham N, Bonham D, Cobb L (eds) Proc 13th World Orchid Conf, 1990 World Orchid Conf Trust, Auckland, New Zealand, pp 284

    Google Scholar 

  • Datla RSS, Hammerlindl JK, Pelcher LE, Crosby WL, Selvaraj G (1991) A bifunctional fusion between ß-glucuronidase and neomycin phosphotransferase: A broad-spectrum marker enzyme for plants. Gene 101: 239–246

    Article  PubMed  CAS  Google Scholar 

  • Dekeyser RA, Claes B, De Rycke RMU, Habets ME, Van Montagu MC, Caplan AB (1990) Transient gene expression in intact and organized rice tissue. Plant Cell 2: 591–602

    PubMed  CAS  Google Scholar 

  • De la Pena A, Lörz H, Schell J (1987) Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature 325: 274–276

    Article  Google Scholar 

  • Dressler RL (ed) (1990) The orchids, natural history and classification, 2nd edn. Harvard Univ Press, Cambridge

    Google Scholar 

  • Duan X, Chen S (1985) Variation of the characters in rice (Oryza sativa) induced by foreign DNA uptake. China Agric Sci 3: 6–9

    Google Scholar 

  • Griesbach RJ (1992) Incorporation of the GUS gene into orchids via embryo electrophoresis. HortScience 27: 620

    Google Scholar 

  • Hawaii Agricultural Statistics Service (1993) Statistics of Hawaiian agriculture 1992. Hawaii Dept of Agriculture, US Dept of Agriculture, Honolulu, Hawaii

    Google Scholar 

  • Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387–405

    Article  CAS  Google Scholar 

  • Jefferson RA, Burgess SM, Hirsh D (1986) β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA 83: 8447–8451

    Article  PubMed  CAS  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907

    PubMed  CAS  Google Scholar 

  • Jenes B, Moore H, Cao J, Zhang W, Wu R (1993) Techniques for gene transfer. In: Kung SB, Wu R (eds) Transgenic plants, vol I. Academic Press, New York, pp 125–146

    Google Scholar 

  • Kamemoto H (1985) Seed-propagated amphidiploid dendrobium cultivars. HortScience 20: 1-2, 163

    Google Scholar 

  • Kamemoto H (1987) Four decades of research on orchid cytogenetics and breeding. In: Saito K, Tanaka R (eds) Proc 12th World Orchid Conf, 1987 World Orchid Conf Trust, Tokyo, Japan, pp 59–73

    Google Scholar 

  • Klein TM, Wolf ED, Wu R, Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73

    Article  CAS  Google Scholar 

  • Knudson L (1922) Nonsymbiotic germination of orchid seeds. Bot Gaz 73: 1–15

    Article  Google Scholar 

  • Kuehnle AR, Sugii N (1992) Transformation of Dendrobium orchid using particle bombardment of protocorms. Plant Cell Rep 11: 484–488

    CAS  Google Scholar 

  • Messeguer J, Martinez Izquierdo JA, Menossi M, Melé E (1993) GUS expression in carnation using particle gun bombardment. In: Schiva T, Mercuri A (eds) Creating genetic variation in ornamentals. Proc XVIIth Eucarpia Symp, Sanremo, Italy, pp 257–261

    Google Scholar 

  • Morel G (1974) Clonal multiplication of orchids. In: Withner CL (ed) The orchids: scientific studies. John Wiley & Sons, Inc, New York, pp 169–222

    Google Scholar 

  • Morikawa H, Iida A, Matsui D, Ikegami M, Yamada Y (1986) Gene transfer into intact plant cells by electroinjection through cell walls and membranes. Genes 41: 121–124

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nan GL, Kuehnle AR (In Press) Factors affecting gene delivery by particle bombardment of Dendrobium orchids. In vitro cell Devel Biol Plant

    Google Scholar 

  • Porter K (1994) Transmission and control of cymbidium mosaic virus during seed and tissue culture propagation. Master Thesis, University of Hawaii at Manoa, Honolulu, Hawaii

    Google Scholar 

  • Russell JA, Roy MK, Sanford JC (1992) Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol 98: 1050–1056

    Article  PubMed  CAS  Google Scholar 

  • Sagawa Y, Israel HW (1964) Post-pollination ovule development in Dendrobium orchids. I. Caryologia 17: 53–64

    Google Scholar 

  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354

    Article  PubMed  CAS  Google Scholar 

  • Senaratna T, McKersie BD, Kasha KJ, Procunier JD (1991) Direct DNA uptake during the imbibition of dry cells. Plant Sci 79: 223–228

    Article  CAS  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Article  PubMed  CAS  Google Scholar 

  • Topfer R, Gronenborn B, Schell J, Steinbiss HH (1989) Uptake and transient expression of chimeric genes in seed-derived embryos. Plant Cell 1: 133–139

    PubMed  CAS  Google Scholar 

  • Vacin E, Went F (1949) Some pH changes in nutrient solution. Bot Gaz 110: 605–613

    Article  CAS  Google Scholar 

  • Withner CL (1943) Ovule culture: a new method for starting orchid seedlings. Am Orchid Soc Bull 11: 261–263

    Google Scholar 

  • Ye GN, Daniell H, Sanford JC (1990) Optimization of delivery of foreign DNA into higher-plant chloroplasts. Plant Mol Biol 15: 809–819

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Horticulture, University of Hawaii, Honolulu, HI, 96822, USA

    G.-L. Nan & A. R. Kuehnle

Authors
  1. G.-L. Nan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. R. Kuehnle
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Nan, GL., Kuehnle, A.R. (1995). Genetic Transformation in Dendrobium (Orchid). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VI. Biotechnology in Agriculture and Forestry, vol 34. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57840-3_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-57840-3_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63374-4

  • Online ISBN: 978-3-642-57840-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation