Abstract
The ability to transfer DNA into living cells has revolutionized the study of biology. Transfection of DNA into mammalian cells is now a widely used technique which, inevitably, has evolved an equally wide number of technical variants. This chapter will describe some of the easiest and most reproducible methods for transferring DNA into a variety of mammalian cells, both those that grow as adherent monolayers and those that grow in suspension.
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© 1996 Springer-Verlag Berlin Heidelberg
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Rolfe, M. (1996). Stable and Transient Transfections in Cell Cycle Studies. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_8
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DOI: https://doi.org/10.1007/978-3-642-57783-3_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58066-9
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