Abstract
A major goal in the study of cell cycle division, as in any area of cell biology, is to define the function of each cellular component and its interplay with other factors. Immunofluorescence microscopy allows the study of the abundance and subcellular localization of any protein throughout the cell cycle, and thus provides important information towards an understanding of their role. This technique combines the sensitivity of fluorescent probes with the spatial resolution of the light microscope and the specificity of antibodies. Several different fluorescent probes can be selectively detected in a mixture of molecules due to their distinct and characteristic excitation and emission wavelengths. By choosing different color fluorophores, simultaneous detection of multiple antigens within a cell is possible providing a direct means of comparing their relative spatial and temporal distribution.
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© 1996 Springer-Verlag Berlin Heidelberg
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Cardoso, M.C., Leonhardt, H. (1996). Immunofluorescence Techniques in Cell Cycle Studies. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_2
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DOI: https://doi.org/10.1007/978-3-642-57783-3_2
Publisher Name: Springer, Berlin, Heidelberg
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