Abstract
In the absence of well defined origins of DNA replication from animal cells, the biochemical approach to study DNA replication as related to the cell cycle, has been confined to two systems. One is the replication of exogenous DNA in Xenopus egg extracts (see chapter by P. Clarke, Part Two), and the other, which is the subject of this chapter, is the in vitro replication of DNA based on the replication of the papovavirus SV40.
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References
Cheng LZ, Workman JL, Kingston RE, Kelly TJ (1992) Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16. Proc Natl Acad Sci USA 89:589–593
D’Urso, G,. Marraccino RL, Marshak DR, Roberts JM (1990) Cell cycle control of DNA replication by a homologue from human cells of the p34Cdc2 protein kinase. Science 250: 786–791
Dutta A, Stillman B (1992) Cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication. Embo J 11:2189–2199
Dutta A, Ruppert JM, Aster JC, Winchester W (1993) Inhibition of DNA replication factor RPA by p53. Nature 365: 9–82
Friedman PN, Kern SE, Vogelstein B, Prives C (1990) Wild-type, but not mutant, human p53 proteins inhibit the replication activities of simian virus 40 large tumor antigen. Proc Natl Acad Sci USA 87:9275–9279
Harlow E, Crawford LV, Pim DC, Williamson NM (1981) Monoclonal antibodies specific for simian virus 40 tumor antigens. J of Virol 39:861–869
Hurwitz J, Dean FB, Kwong AD, Lee S-H (1990) The in vitro replication of DNA containing the SV40 origin. J Biol Chem 265:18043–18046
Kelly TJ (1988) SV40 DNA replication. J Biol Chem 263:17889–17892
Laybourn PJ, Kadonaga JT (1991) Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II. Science 254:238–45
Roberts JM, D’Urso G (1988) An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle. Science 241:1486–1489
Stillman B (1989) Initiation of eukaryotic DNA replication in vitro. Ann Rev Cell Biol 5: 197–245
Virshup, DM, Kauffman MG, Kelly TJ (1989) Activation of SV40 DNA replication in vitro by cellular protein-phosphatase 2A. Embo J 8:3891–3898
Waga S, Hannon G, Beach D, Stillman S (1994) The p21 inhibitor of Cyclin dependent kinases controls DNA replication by interaction with PCNA. Nature 369:574–578
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© 1996 Springer-Verlag Berlin Heidelberg
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Dutta, A., Winchester, E. (1996). SV40-Based in Vitro DNA Replication Assay. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_16
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DOI: https://doi.org/10.1007/978-3-642-57783-3_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58066-9
Online ISBN: 978-3-642-57783-3
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