Summary
The genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells.
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© 2000 Springer-Verlag Berlin Heidelberg
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Just, U., Boettiger, D., Kan, O., Dexter, T.M., Spooncer, E. (2000). Insertional Mutagenesis as a Route to Identifying Genes Involved in Self Renewal of Haemopoietic Stem Cells. In: Melchers, F. (eds) Lymphoid Organogenesis. Current Topics in Microbiology and Immunology, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57276-0_4
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DOI: https://doi.org/10.1007/978-3-642-57276-0_4
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