Abstract
We describe nonradioactive in situ hybridization (ISH) approaches for localization of viral DNA and RNA sequences in infected tissue. The use of non-isotopic ISH methods has become common because they are now as sensitive as autoradiography but they do not have the the disadvantages of the isotopic methods, such as the long assay time and the requirement for special laboratory facilities (Musiani et al., 1994).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Egger D, Troxler M, Bienz K (1994) Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem Cytochem 42:815–822
Erälinna J-P, Soilu-Hänninen M, Röyttä M, Hukkanen V, Salmi A, Salonen R (1996) Blood-brain barrier breakdown and increased intercellular adhesion molecule (ICAM-1/CD54) expression after Semliki Forest (A7) virus infection facilitates the development of experimental allergic encephalomyelitis. J Neuroimmunol 66:103–114
Furuta Y, Shinohara T, Sano K, Meguro M, Nagashima K (1990) In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 43:806–809
Heino P, Hukkanen V, Arstila P (1989) Detection of human papilloma virus (HPV) DNA in genital biopsy specimens by in situ hybridization with digoxigenin-labeled probes. J Virol Methods 26:331–338
Hukkanen V, Heino P, Sears A E, Roizman B (1990) Detection of herpes simplex virus latency-associated RNA in mouse trigeminal ganglia by in situ hybridization using nonradioactive digoxigenin-labeled DNA and RNA probes. Methods Mol Cell Biol 2:70–81
Maples JA (1985) A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays. Am J Clin Pathol 83:356–363
McQuaid S, McMahon J, Allan GM (1995) A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization. Biotech Histochem 70:147–154
Morris RG, Arends MJ, Bishop PE, Sizer K, Duvall E, Bird CC (1990) Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation. J Clin Pathol 43:800–805
Musiani G, Gentilomi G, Zerbini M, Gibellini D, Gallinella G, Pileri S, Baglioni P, La Placa M (1990) In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry 94:21–25
Musiani M, Zerbini M, Gibellini D, Venturoli S, Gentilomi G, Gallinella G, La Placa M (1994) Viral diagnosis using hybridization assays with digoxigenin labeled probes. Clin Chim Acta 226:237–245
Permeen AMY, Sam CK, Pathmanathan R, Prasad U, Wolf H (1990) Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigeninlabelled probe. J Virol Methods 27:261–268
Ramakrishnan R, Levine M, Fink DJ (1994) PCR-based analysis of herpes simplex virus type 1 latency in the rat trigeminal ganglion established with a ribonucleotide reductase-deficient mutant. J Virol 68:7083–7091
Wu T-C, Mann RB, Epstein JI, MacMahon E, Lee WA, Charache P, Hayward SD, Kurman RJ, Hayward GS, Ambinder RF (1991) Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. Am J Pathol 138:1461–1469
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Hukkanen, V., Heino, P. (2000). Virus Detection in Biopsy Specimens. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_45
Download citation
DOI: https://doi.org/10.1007/978-3-642-57206-7_45
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
eBook Packages: Springer Book Archive