Abstract
The analysis of proteins on blots after electrophoretic transfer from gels rather than analyzing the gels themselves is becoming increasingly popular due to several advantages, e.g., application of immunological techniques (“classical western blotting”); membranes do not shrink and are easy to handle; and several types of membranes with different advantages are available. However, general protein staining techniques of blots are by far not as well established as the very sensitive silver staining method of gels. Staining techniques using gold probes are approaching this sensitivity and are very useful [1]. By adapting the immunological digoxigenin (DIG)/anti-digoxigenin:alkaline phosphate (<DIG>:AP) system for the general staining of proteins on blots, we developed a second very sensitive system which should be even more useful due to its greater flexibility and convenient combination with other immunological detection methods of blots.
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© 2000 Springer-Verlag Berlin Heidelberg
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Haselbeck, A., HÖSel, W. (2000). Detection of Proteins and Glycoproteins on Western Blots. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_41
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DOI: https://doi.org/10.1007/978-3-642-57206-7_41
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
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