Abstract
Due to the high specificity of the mature polyclonal anti-DIG antibody, isolated from sheep, even structurally related steroids such as the bufadienolide k-strophanthin (cross-reactivity 0.1%) or the sex and suprarenal steroids (cross-reactivity<0.0003%) show only minor side reactions. In order to avoid nonspecific binding reactions of the Fc portion of the antibody, the Fab fragment of the DIG-specific antibody is used as the binding component when only primary antibodies are applied. The entire DIG-specific antibody, isolated from sheep including the Fc portion is used for signal generation by secondary antibodies. Labeled mouse antibodies whose specificity is directed against the Fc portion of sheep antibodies serve as secondary antibodies in this case.
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References
Kessler C (1991) The digoxigenin (DIG) technology - a survey on the concept and realization of a novel bioanalytical indicator system. Mol Cell Probes 5:161–205
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© 2000 Springer-Verlag Berlin Heidelberg
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Kessler, C. (2000). Overview on the Digoxigenin: Anti-Digoxigenin (DIG) System. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_3
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DOI: https://doi.org/10.1007/978-3-642-57206-7_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
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