Abstract
The polymerase chain reaction (PCR) is a powerfulin vitromethod in molecular biology for selective, highly specific and exceptionally efficient amplification of nucleic acid sequences. In the 10 years since the first publication on PCR (Saiki et al., 1985) this method has grown to rival in popularity traditional microbiological, genetic and technical procedures for cloning, sequencing, gene detection and related procedures. Furthermore, in the meantime PCR and all of its different applications are rapid and convenient alternatives to traditional procedures such as blotting technologies, conventional hybridization and molecular cloning. Initially, PCR was a rather complex and tricky generic procedure applied to basic research problems in molecular biology. It has developed into a simple, multipurpose procedure more or less optimized for diverse applications in nearly every biological discipline and commercial area. There are frequent instances of PCR techniques having passed into the service laboratory environment. These service laboratories are providing a broad range of diagnostic tests mainly covering medical and forensic applications, but also environmental, agricultural and veterinary topics.
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Rolfs, A., Finckh, U., Bauer, P. (2000). Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Principles and Applications. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_25
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DOI: https://doi.org/10.1007/978-3-642-57206-7_25
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