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Labeling of Biomolecules with Fluorophores

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Nonradioactive Analysis of Biomolecules

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Abstract

Coumarins, fluorescein, and resorufin derivatives are three important fluorescence labels which can be used for labeling of biomolecules. During the last years a wide range of new fluorescence labels have been published and are commercially available, especially covering the long wavelength/ NIR range. Among these are new rhodamine and oxazine derivatives (Arden-Jakob et al., 1997) cyanine derivatives (Thompson, 1994) and also BODIPY fluorophores (Haugland, 1996). The development was especially driven by the upcoming cheap laser diodes (Wersig et al., 1993) and the demand to reduce background fluorescence.

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References

  • Arden-Jakob J, Marx NJ, Drexhage KH (1997) New Fluorescent Probes for the red spectral region. Journal of Fluorescence 7/1 (Supplement): 91S–93S

    Google Scholar 

  • Bernard PS, Lay MJ, Wittwer CT (1997), Integrated Amplification and Detection of the C677T Point Mutation by Fluorescence Resonance Energy Transfer. Analytical Biochemistry 254

    Google Scholar 

  • Haugland EP (1983) Covalent fluorescent probes. In: Steiner RF (ed) Excited States of Biopolymers. Plenum Press, New York, London, pp 29–58

    Book  Google Scholar 

  • Haugland EP (1996) Handbook of Fluorescent Probes and Research Chemicals, Sixth Edition. Molecular Probes, Eugene, OR

    Google Scholar 

  • Hemmilä I (1985) Fluoroimmunoassays and immunofluorometric assays. Clinical Chemistry 31:359–370

    PubMed  Google Scholar 

  • Herrmann R, Josel H-P, Wörner W, Fetterhof TJ (1989) Conjugation of proteins to various fluorescence labels, 19th FEBS meeting, Rom, Abstract Nr FR 511

    Google Scholar 

  • Klein C, Batz H-G, Draeger B, Guder H-G, Herrmann R, Josel H-P, Nägele U, Schenk R, Vogt B (1993) Fluorescence Polarisation Immunoassay. In: Fluorescence Spectroscopy, Wolfbeis OE (ed). Springer-Verlag, Berlin/Heidelberg.

    Google Scholar 

  • Miller JN (1993) Long wavelength fluorescence spectroscopy. Spectroscopy Europe 5/ 2:34–38

    Google Scholar 

  • Nederlof PM, van der Flier S, Wiegant J, Raap AK, Tanke HJ, Ploem JS, van der Ploeg M (1990) Multiple fluorescence inin situhybridization. Cytometry 11: 126–131

    Article  PubMed  CAS  Google Scholar 

  • Raffael A (1988) GIT Labor-Medizin, 89–97

    Google Scholar 

  • Rypacek F, Drobnik J, Katal J (1980) Fluorescence labeling method for estimation of soluble polymers in living material. Analytical Biochemistry 104:141–149

    Article  PubMed  CAS  Google Scholar 

  • Sauer M, Zander C, Müller R, Ullrich B, Drexhage KH, Kaul S, Wolfrum J (1997) Detection and identification of individual antigen molecules in human serum with pulsed semiconductor lasers. Applied Physics B 65: 427–431

    Article  CAS  Google Scholar 

  • Shapiro HM (1988) Practical Flow Cytometry. Alan R. Liss, Inc. New York

    Google Scholar 

  • Thompson RB (1994) Red and Near-Infrared Fluorimetry. In: Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing. Lakowicz JR, Plenum Press, New York

    Google Scholar 

  • Wersig C, Finke W, Händler E, Josel H-P, Schmidt E (1993) Performance of commercial laser diodes in fluorometric detection. Advances in Fluorescence Sensing Technology 1885:389–400

    Article  Google Scholar 

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© 2000 Springer-Verlag Berlin Heidelberg

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Heindl, D., Josel, HP. (2000). Labeling of Biomolecules with Fluorophores. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_22

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  • DOI: https://doi.org/10.1007/978-3-642-57206-7_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-64601-3

  • Online ISBN: 978-3-642-57206-7

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