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Overview on Nonradioactive Labeling Systems

  • Chapter
Nonradioactive Analysis of Biomolecules

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

A variety of labeling systems for nucleic acids, proteins, and glycans has been developed in the past decade.

Table 1 shows an overview on important labeling systems including cross reference to the description in Part A. For surveys on nonradioactive labeling systems see Duering (1993); Jenkins (1994); Mansfield et al. (1995); Cunningham et al., (1995); Kessler (1992a; 1994; 1995) Hoeltke et al. (1995); Savage (1996); McCreery (1997). Among the indirect approaches the most sensitive systems are the biotin:(strept-)avidin (bio)system and the digoxigenin:anti-digoxigenin (DIG) system detecting less than picogram levels of DNA or RNA. However, the other indirect systems are also of interest, especially in particular applications like study of cell proliferation (Br-dU system)in situstudies (sulfone system), or staining of tissue sections in addition to histological staining (immunogold system). The direct AP- or HRPbased systems described are useful, e.g., for hybridization with standard probes as used in fingerprinting or membrane-based sequencing approaches.

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  1. Roche Diagnostics GmbH, Roche Molecular Systems, Bahnhofstrasse, 9–15, Tutzing, 82327, Germany

    Christoph Kessler

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  1. Christoph Kessler
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  1. Roche Molecular Systems Werk Tutzing, Roche Diagnostics GmbH, Bahnhofstrasse 9-15, 82327, Tutzing, Germany

    Christoph Kessler

  2. Gene Center/Institute of Biochemistry, Ludwig-Maximilians-Universität München, Feodor-Lynen-Str. 25, 81377, München, Germany

    Christoph Kessler

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© 2000 Springer-Verlag Berlin Heidelberg

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Kessler, C. (2000). Overview on Nonradioactive Labeling Systems. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-57206-7_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-64601-3

  • Online ISBN: 978-3-642-57206-7

  • eBook Packages: Springer Book Archive

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