Abstract
In order to detect biomolecules such as nucleic acids or proteins on blots orin situenzyme-linked immunoassays are used. In most of these applications, alkaline phosphatase (AP) is the enzyme of choice. AP is either conjugated to an antibody or to streptavidin which then binds to specific antigens or digoxigenin-fluorescein-or biotin-labeled target molecules, respectively. In all these examples, AP can be visualized by its ability to catalyze specific color reactions. Conventionally, BCIP and NBT are employed as substrates yielding a blue precipitate when the phosphoryl group is released (McGadey, 1970). Here, we describe another substrate for AP that presents several advantages compared to the BCIP/NBT system: naphthol AS phosphates together with diazonium salts (Fast salts).
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© 2000 Springer-Verlag Berlin Heidelberg
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SchÜssler, P., Grevelding, C.G., Kunz, W. (2000). Azo Dyes. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_17
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DOI: https://doi.org/10.1007/978-3-642-57206-7_17
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
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