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Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods pp 127–141Cite as

Protein Blotting and Immunoblotting

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Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

Immunoblotting was first devised by Towbin et al. (1979) to exploit the specificity of the reaction between antibodies and proteins transferred from SDS- polyacrylamide electrophoretic gels onto nitrocellulose. Immunoreactive bands were evidenced by labelled “second antibody” or protein A.

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Author information

Authors and Affiliations

  1. Dipartimento Biologia Molecolare, Università degli Studi di Siena, Via Fiorentina 1, 53100, Siena, Italy

    L. Bini, S. Liberatori, B. Magi, B. Marzocchi, R. Raggiaschi & V. Pallini

Authors
  1. L. Bini
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  2. S. Liberatori
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  3. B. Magi
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  4. B. Marzocchi
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  5. R. Raggiaschi
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  6. V. Pallini
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Editor information

Editors and Affiliations

  1. CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, CEA-Grenoble, 17 rue des Martyrs, 38054, Grenoble Cedex 9, France

    Thierry Rabilloud

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© 2000 Springer-Verlag Berlin Heidelberg

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Bini, L., Liberatori, S., Magi, B., Marzocchi, B., Raggiaschi, R., Pallini, V. (2000). Protein Blotting and Immunoblotting. In: Rabilloud, T. (eds) Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57105-3_6

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  • DOI: https://doi.org/10.1007/978-3-642-57105-3_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-65792-7

  • Online ISBN: 978-3-642-57105-3

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