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Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods pp 31–55Cite as

Two-Dimensional Electrophoresis with Carrier Ampholytes

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Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

Two-dimensional (2-D) gel electrophoresis, which was originally described by O’Farrell (1975), separates proteins in the first dimension according to their isoelectric point, and in the second dimension according to their molecular weight. It offers the opportunity of separating several hundred proteins from a total cellular extract. In combination with the recent development of methods of protein identification based on microsequencing, amino acid composition and mass spectrometry, the technique of 2-D gel electrophoresis now provides an invaluable tool for proteomic studies.

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Author information

Authors and Affiliations

  1. Institut de Biochimie et Génétique Cellulaires, UPR CNRS S9026, 1, rue Camille Saint-Saëns, 33700, Bordeaux Cedex, France

    C. Monribot & H. Boucherie

Authors
  1. C. Monribot
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  2. H. Boucherie
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Editor information

Editors and Affiliations

  1. CEA-Laboratoire de Bioénergétique Cellulaire et Pathologique EA 2019, DBMS/BECP, CEA-Grenoble, 17 rue des Martyrs, 38054, Grenoble Cedex 9, France

    Thierry Rabilloud

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© 2000 Springer-Verlag Berlin Heidelberg

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Monribot, C., Boucherie, H. (2000). Two-Dimensional Electrophoresis with Carrier Ampholytes. In: Rabilloud, T. (eds) Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57105-3_3

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  • DOI: https://doi.org/10.1007/978-3-642-57105-3_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-65792-7

  • Online ISBN: 978-3-642-57105-3

  • eBook Packages: Springer Book Archive

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