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The Assembly of Recombinant Signaling Systems and Their Use in Investigating Signaling Dynamics

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The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 148))

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Abstract

Recombinant signaling systems are a basic component of research efforts concerned with the structure and function of members of the superfamily of membrane receptors coupled to heterotrimeric G proteins. The widespread use of such systems in pharmacology began in the mid 1980s following isolation of the complementary DNAs (cDNAs) encoding β-adrenergic receptors (ARs) and muscarinic receptors. The next decade witnessed the determination of the primary structure of several protein families involved in signal propagation, including receptors, G proteins and effectors. In the midst of this activity, transient and stable expression systems were used to determine the ligand-recognition properties and functional domains of the receptor protein in addition to the signal-transduction events initiated by agonist-activated receptors. Generally, this approach involved ectopic expression of the receptor in a mammalian cell line. With recent advances in gene technology, recombinant systems can also be generated in Saccharomyces cerevisae, aplysia, Dictyostelium discoideum, Caenorhabditis elegans, Drosophila melanogaster, transgenic rodents and mini-swine.

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  1. S. M. Lanier
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Editors and Affiliations

  1. Department of Receptor Biochemistry, Glaxo Wellcome Research and Development, 5 Moore Drive, Research Triangle Park, 27709, NC, USA

    Terry Kenakin Ph.D. (Principal Research Scientist) (Principal Research Scientist)

  2. Department of Pharmacology, The University of Melbourne, 3010, Parkville, VIC, Australia

    James A. Angus Ph.D. FAA (Head) (Head)

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Lanier, S.M. (2000). The Assembly of Recombinant Signaling Systems and Their Use in Investigating Signaling Dynamics. In: Kenakin, T., Angus, J.A. (eds) The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems. Handbook of Experimental Pharmacology, vol 148. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57081-0_12

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  • DOI: https://doi.org/10.1007/978-3-642-57081-0_12

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