Abstract
First, what is a data file? A flow cytometry data file is the compilation of measurements from the acquisition of a single sample on a flow cytometer. A data files standard is essentially a list of rules describing how the computer data file should be organized. When adhered to, the rules yield a defined and uniform computer file structure. The letters ‘FCS’ refer specifically to the Flow Cytometry Standard format, which is a comprehensive data-file structure with a 16 year history of use in flow cytometry.
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References
Murphy RF, Chused TM: A proposal for a flow cytometric data file standard. Cytometry 1984; 5:553–555.
Dean PN, Bagwell CB, Lindmo T, et al. Data File Standard for Flow Cytometry. Cytometry 1990; 11:323–332.
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© 2000 Springer-Verlag Berlin Heidelberg
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Seamer, L. (2000). Flow Cytometry Standard (FCS) Data File Format. In: Diamond, R.A., Demaggio, S. (eds) In Living Color. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57049-0_6
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DOI: https://doi.org/10.1007/978-3-642-57049-0_6
Publisher Name: Springer, Berlin, Heidelberg
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